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Srinivas Kiran Ambatipudi
Associate Professor
Research Interests

Educational Details
Professional Background


Honours and Awards

Teaching Engagements
Teaching Engagements

Associate Scholars

Administrative Positions
Research Interests
Application of proteomics to elucidate the role of milk proteins in the pathophysiology of animal disease, Chemoresistance and breast cancer progression
Educational Details
Macquarie University, Sydney, Australia
PhD, Biological Sciences
University of Birmingham, Birmingham, UK
MSc, Applied Genetics
Odisha University of Agriculture and Technology
BVSc. & AH, Veterinary Science
Professional Background
12 Aug 2013 - 15 Jan 2014
Vaccine Production Program Laboratory, National Institutes of Health, Bethesda, USA
Senior Scientist
24 Jun 2013 - 09 Aug 2013
Eurofins Medinet, Chantilly, USA
Research Fellow
21 Jun 2010 - 15 Feb 2013
Dental and Craniofacial Institute, National Institutes of Health, Bethesda, USA
Postdoctoral Research Associate
15 Aug 2007 - 18 Jun 2010
School of Medicine and Dentistry, University of Rochester, Rochester, USA
01 Jan 2005 - 01 Jan 2007
School of Biological Sciences, Macquarie University, Sydney, Australia
Climate Smart Buffalo Farming using Digital Smart System 01 Apr 2024 Veterinary Science, Sensor Technology and Artificial Intelligence Extramural Funding Bill and Melinda Gates Foundation
Cow Infrared Analytical System for early diagnosis of Subclinical Mastitis 01 Oct 2023 Veterinary Science and Artificial Intelligence Extramural funding Sree Padmavathi Venkateswara Foundation
Bovine Milk Fat Globule Characterization 05 Aug 2020 Veterinary Science -Grant Approved Department of Biotechnology, Govt of India
Bee sting 07 Jun 2021 Therapeutics Extramural Funding Department of Biotechnology, Govt of India
Bovine Milk Glycoproteomics 04 Aug 2016 Veterinary Science Extramural Funding Department of Science and Technology, Govt of India
Lipidomics of Bovine Milk Under Different Seasons and Feeds 04 Sep 2015 Veterinary Science -Extramural Funding Department of Biotechnology, Govt of India
Quantitative Proteomics of Milk Proteins in Bovine Mastitis 01 Apr 2014 Veterinary Science Intramural Funding IITR

Hidden Treasure: Veterinary Science
Year: 2021 , ISBN: 978-93-90757-07-7
Editors: Debiprasanna Das, Kiran Ambatipudi, Bhabesh C. Das, Basanti Jena | Brillion Publishing
Pages: 314 ,


1Ayushi Kapoor, Saurav Datta, Gaurav Gupta, Ajay Vishwakarma, Avinash Singh, Sujay Chattopadhyay, Kiran Ambatipudi. One-step separation of fat globules based on size from bovine milk using a cross-flow microfiltration. Separation and Purification Technology, 330, Part A, 125251.


2. Arpita Das, Rama N. Behera, Ayushi Kapoor, Kiran Ambatipudi. The potential of meta-proteomics and artificial intelligence to establish the next generation of probiotics for personalized healthcare. Journal of Agricultural and Food Chemistry, 71, 17528-17542.

3.Shubham Jaina, Ankita Bhatt, Shahnawaz Ahmad Baba, Vinod Singh Bisht, Pinakshi Biswas, Kiran Ambatipudi, Naveen Kumar Navani. Concurrent mitigation and facile monitoring of xenobiotics by a highly efficient and recyclable nanoengineered catalyst. Chemical Engineering Journal, 145074

4. Rama N. Behera, Vinod S. Bisht, Kuldeep Giri, Kiran AmbatipudiRealm of proteomics in breast cancer management and drug repurposing to alleviate intricacies of treatment. Proteomics - Clinical Applications, e2300016.

5.Ayushi Kapoor, Aparna Verma, Kiran AmbatipudiCharacterization of cow, goat, and water buffalo milk fat globule lipids by high-performance thin layer chromatography. Dairy, 200-214.


6. Arpita Das, Kuldeep Giri, Rama N. Behera, Sudipa Maity and Kiran Ambatipudi. BoMiProt 2.0: An update of bovine milk proteins database. Journal of Proteomics, 267, 104696.

7. Kuldeep Giri, Sudipa Maity and Kiran Ambatipudi. Targeted proteomics using parallel reaction monitoring confirms salivary proteins indicative of metastatic triple-negative breast cancer. Journal of Proteomics, 267, 104701. Highlighted in Nature India

8. Monica Yadav, Ayushi Kapoor, Aparna Verma, Kiran Ambatipudi. Functional significance of different milk constituents in modulating gut microbiome and infant health. Journal of Agricultural and Food Chemistry, 70, 3929-3947. 

9. Ana Caroline Costa-da-Silva, Marit H Aure, Joshua Dodge, Daniel Martin, Susan Dhamala, Monica Cho, Jeremy J Rose, Carol W Bassim, Kiran Ambatipudi, Frances T Hakim, Steven Z Pavletic, Jacqueline W Mays . Salivary ZG16B expression loss follows exocrine gland dysfunction related to oral chronic graft-versus-host disease. iScience, 25, 103592.

10. Shweta Jha*, Sudipa Maity, Jahawar Singh, Chaya Chouhan, Nisha Tak, Kiran Ambatipudi.  Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt-responsive mechanisms. Physiologia Plantarum, 174, e103592. *Corresponding Author


11.Neetu Neetu, Madhusudhanarao Katiki, Krishna Mahto, Monica Sharma, Anoop Narayanan, Sudipa Maity, Shailly Tomar, Kiran Ambatipudi, Ashwani Kumar Sharma, Dinesh Yernool, Pravindra Kumar. Deciphering the enigma of missing DNA bnding domain of LacI family transcription factors. Archives of Biochemistry and Biophysics. 713, 30.

12.Vinod S. Bisht, Kuldeep Giri, Deepak Kumar, Kiran Ambatipudi. Oxygen and Metabolic Reprogramming in the Tumor Microenvironment Influences Metastasis Homing. Cancer Biology & Therapy, 25, 1-20.

13.Kuldeep Giri, Sudipa Maity, Kiran Ambatipudi. In Silio Data Mining of Human Body Fluids to Unravel the Immunome in Breast Cancer. Journal of Proteins and Proteomics, 12, 45-62.

14. Sudipa Maity and Kiran AmbatipudiMammary Microbial Dysbiosis Leads to the Zoonosis of Bovine Mastitis: A One-Health Perspective. FEMS Microbiology Ecology, 97 (1).


15.Aparna Verma, Ningombam Sanjib Meitei, Prakash Gajbhiye, Mark Raftery, Kiran Ambatipudi. Comparative analysis of milk triglycerides profile between Jafarabadi buffalo and Holstein Friesian cow. Metabolites,10 (12), 507.

16.Sudipa Maity, Debiprasanna Das, Kiran AmbatipudiQuantitative Alterations in Bovine Milk Proteome between  Healthy, Subclinical and Clinical Mastitis during S. aureus Infection. Journal of Proteomics,  223, 103815. 

17.Sudipa Maity, Aadil Bhat, Kuldeep Giri, Kiran Ambatipudi. BoMiProt: A Database of Bovine Milk Proteins. Journal of Proteomics, 215, 103648.

18. Aparna Verma, Ajay K. Sharma, Ayushi Agarwal, Saurav Datta*, Kiran Ambatipudi*. Selective Enrichment of Milk Fat Globules Using Functionalized Polyvinylidene Fluoride membrane. Preparative Biochemistry and Biotechnology, 50, 18-27. ¶ Equal Contribution; * Co-Corresponding Author


19.Aparna Verma, Tamoghna Ghosh, Bharat Bhushan, Gopinath Packirisamy, Naveen K. Navani, Pranita P. Sarangi, Kiran Ambatipudi. Characterization of Difference in Structure and Function of Fresh and Mastitic Bovine Milk Fat Globules. PLoS One, 14 (8), e0221830.

20.Teotia D, Gaid M, Saini SS, Verma A, Yennamalli RM, Khare SP, Ambatipudi K, Mir JI, Beuerle T, Hänsch R, Roy P, Agrawal PK, Beerhues L, Sircar D. Cinnamate:CoA-ligase is involved in Benzoate-derived Biphenyl Phytoalexin Biosynthesis in Malus × domestica 'Golden Delicious' Cell Cultures. Plant J. 100, 1176-1192.

21.Sudipa Maity, Kiran AmbatipudiQuantitative Proteomics of Milk Whey Reveals Breed and Season Specific Variation in Protein Abundance in Holstein Friesian Cow and Murrah Buffalo. Research in Veterinary Science, 125, 244-252.

22.Kuldeep Giri, Anurag Mehta, Kiran Ambatipudi. In search of the Altering Salivary Proteome in Metastatic Breast and Ovarian Cancer. FASEB BioAdvances, 1, 191-207. (In the top 10% of most downloaded papers  of FASEB BioAdvances Journal between January 2018-December 2019)

23.Aadil H. Bhat, Sudipa Maity, Kuldeep Giri, Kiran Ambatipudi. Protein glycosylation: Sweet or Bitter for Bacterial Pathogens? Critical Reviews in Microbiology, 45, 82-102.


24. Atin Sharma, Vineet Dubey, Rajnikant Sharma, Kuldip Devnath, Vivek Kumar Gupta, Jawed Akhter, Timsy Bhando, AparnaVerma, Kiran Ambatipudi, Mihir Sarkar, Ranjana Pathania. The unusual glycine rich C-terminus of the Acinetobacter baumannii RNA chaperone Hfq plays an important role in bacterial physiology. Journal of Biological Chemistry, 293, 13377-13388.

25. #Sarangi PP,  #Chakraborty P, Dash SP, Ikeuchi T, Vega SD, Ambatipudi K, Wahl L, *Yamada Y. Cell adhesion protein Fibulin-7 and its C-terminal fragment negatively regulate monocyte and macrophage migration and functions in vitro and in vivo. FASEB Journal, 32, 4889-4898.


26Kumar P, Kesari P, Dhindwal S, Choudhary A.K., Katiki M, Neetu, Verma A, Ambatipudi K, Tomar S, Sharma A.K., Mishra G, Kumar P. A novel function for globulin in sequestering plant hormone: Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin. Scientific Reports, 7, 4705.


27.Aparna Verma and Kiran Ambatipudi. Challenges and opportunities of bovine milk analysis by mass spectrometry. Clinical Proteomics, 13, 8. (Invited Review)


28. Shveta Bathla#; Preeti Rawat#; Rubina Baithalu; Munna Lal Yadav; Jasmine Naru; Anurag Tiwari; Sudarshan Kumar; Ashok K. Balhara; Surender Singh; Suman Chaudhary; Rajesh KumarPradip Behere; Sushil K. Phulia; Tushar K. Mohanty; Jai K. Kaushik; Shivramaiah Nallapeta; Inderjeet Singh; Srinivas K. Ambatipudi; Ashok K. Mohanty. Profiling of cow urinary proteins using various extraction methods reveals more than 1550 proteins. Journal of Proteomics, S1874-3919, 30023-3. # (Equal Contribution).



1.  Peter Burbelo, Kiran Ambatipudi, Ilias Alevizos (2014). Genome-wide association studies in Sjögren’s Syndrome: what are the genes telling us about disease pathogenesis? Autoimmunity Reviews, 13, 756-761.

2. Kiran S. Ambatipudi* and James E. Melvin (2013). Cancer biomarker discovery in saliva by mass spectrometry.  (*Corresponding author), Oncology, Gastroenterology and Hepatology Reports, 2, 1-8.

3. Kiran S. AmbatipudiSteve Swatkoski, James J. Moresco, Patricia G. Tu, Andreea Coca, Jennifer H. Anolik, Marjan Gucek, Igñacio Sanz, John R. Yates III, James E. Melvin (2012). Quantitative proteomics of human parotid saliva in primary Sjögren’s syndrome. Proteomics, 12, 1-8.

4.  Bassim CW*, Ambatipudi KS*, Mays JW, Edwards DA, Swatkoski S, Fassil H, Bird K, Gucek M, Melvin  JE, Pavletic SZ (2012). Quantitative salivary proteomics of oral chronic Graft-versus-Host Disease in humans. (*Co- First Author), Journal of Clinical Immunology, 32, 1390-9.

5.  Gregoire S, Silva BB, Gonzalez I, Agidi PS, Xiao J, Klein MI, Ambatipudi KS, Bauserman R, Waugh R, Koo H  (2011). A novel role for the glucosyltransferase B in Candida albicans interactions with Streptococcus mutans and apatitic surfaces. Applied and Environmental Microbiology77, 6357-67.

6.  Kiran S. Ambatipudi, Fred K. Hagen, Claire Delahunty, Xuemei Han, Rubina Shafi, Jennifer Hryhorenko, Stacy Gregoire, Bob Marquis, James E. Melvin, Hyun M. Koo, John R Yates III (2010). Human Common Salivary Protein-1  (CSP-1) Promotes binding of Streptococcus mutans to experimental salivary pellicle and glucans formed on hydroxyapatite surface. Journal of Proteome Research, 9, 6605-14.

7Kiran S. Ambatipudi, Bingwen Lu, Fred K. Hagen, James E. Melvin, John R. Yates III (2009). Quantitative analysis of age specific variation in the expression of human female parotid salivary proteins. Journal of Proteome Research, 8, 5093-5102. (Highlighted in the issue of J Proteome Res.).

8Kiran S. Ambatipudi and Elizabeth Deane (2008). Proteomic analysis of neutrophil granule proteins in the Tammar wallaby (Macropus eugenii). Molecular Immunology, 45; 690-700.

9.  Kiran S. Ambatipudi, Janice Joss, Mark Raftery and Elizabeth Deane (2008). A proteomic approach to analysis of antimicrobial activity in marsupial pouch secretions. Developmental and Comparative Immunology, 32; 108-20.

10.  Kiran S. Ambatipudi, Janice Joss and Elizabeth Deane (2007). A comparative proteomic analysis of skin secretion of the tammar wallaby (Macropus eugenii) and the wombats (Vombatus ursinus). Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, (2); 322-331.

11. Kiran S. Ambatipudi, Julie Old, Michael Guilhaus, Mark Raftery, Lyn Hinds and Elizabeth Deane (2006). Proteomic analysis of neutrophil proteins in the Tammar wallaby (Macropus eugenii). Comparative Biochemistry and PhysiologyPart D: Genomics and Proteomics, (1): 283- 291.

12.  Pattanaik T. K., Nath I., Bose V. S. C., Kiran A. S. (2001). Bone plating of distal radioulnar fracture in a  dog. Indian   Journal of Veterinary SurgeryVol. 22(1): 59-60.

13.  Pattanaik T. K., Nath I., Bose V. S. C., Kiran A. S. (2001). Open reduction of elbow dislocation in a dog. Indian Journal of Veterinary Surgery, Vol. 22(1): 63.




1. Srinivas Kiran Ambatipudi and Kuldeep Giri. A system and method for identification of peptides from saliva to detect triple-negative breast cancer Provisional Patent No: IITRCRN194.

2. Srinivas Kiran Ambatipudi, Saurav Datta, Aparna Verma.“Functionalized membrane-based Process for selective isolation and Enrichment of milk fat globules (MFGs)”. Patent No: 357223

3. Srinivas Kiran Ambatipudi and Aparna Verma. ‘A Bioconversion Process To Produce Bioactive Cordyceps Species From Food Waste’ Indian Patent, Provisional Patent no: 201811023344

Honors And Awards
National Academy of Dairy Science, India
Venus International Foundation
Outstanding Research in Biotechnology (Veterinary and Animal Science Discipline)
Swami Rama Himalayan University, Jolly Grant, Dehradun
Distinguished member of "Research Committee"
Food Safety and Standards Authority of India, Ministry of Health and Family Welfare
Member for "Scientific Panel of Antibiotic Residues"
65th American society of mass spectrometry, Indianapolis, USA
International travel support(DST-SERB)
International Society for Developmental and Comparative Immunology, Charleston, USA
Travel Fellowship for Oral Presentation
Macquarie University, Sydney, Australia
Awarded with Travel Fellowship in the year 2006 during the work at Macquarie University Travel Grant
Australian Peptide Conference, Hamilton Island, Australia
Travel Fellowship for Poster Presentation
International Society for Wild Life Disease, Cairns, Australia
Travel Fellowship for Oral Presentation
International Conference in Genomics and Proteomics
2nd Prize in Oral Presentation
Lorne Protein Conference, Philip Island, Australia
Travel Fellowship for Poster Presentation
American Society of Microbiology
25 Jan 2023 - 24 Jan 2024
Association of Mastitis
01 Apr 2022 - Present
Life member
Proteomics Society of India
10 Dec 2014 - Present
Life Membership
Odisha Veterinary Council
04 Aug 2020 - Present
Teaching Engagements
Teaching Engagements
Genomics and Proteomics ( BTN-441 )
Good Laboratory Practices and Bioethics ( BTN-636 )
Technical Communication ( BTN-524 )
Animal Biotechnology ( BTN-305 )
Technical Communication ( BTN-391 )
Isolation and Characterization of Bovine Milk Lipids
15 Jul 2014 - 09 Sep 2019
Other Supervisors: , Scholar: Aparna Verma
Bovine milk characterization in clinical mastitis
04 Jan 2016 - 12 May 2020
Other Supervisors: , Scholar: Sudipa Maity
Human Salivary Proteomics in Breast Cancer
04 Jan 2016 - 12 Jul 2022
Other Supervisors: , Scholar: Kuldeep Giri
Associate Scholars
Monica Yadav
Indian Institute of Technology Roorkee
Human Milk and its Role in Modulation of Gut Microbiota
Ayushi Kapoor
Indian Institute of Technology Roorkee
Bovine Milk in Breast Cancer Therapy
Vinod Bisht
Indian Institute of Technology Roorkee
Development of Chemosresistance in Breast Cancer
Arpita Das
Indian Institute of Technology Roorkee
Characterization of bovine milk
Rama Behera
Indian Institute of Technology Roorkee
Human Breast Cancer Progression
Career Symposium 2023 by International Faculty for the Trainees in National Institutes of Health
08 May 2023 - 10 May 2023
National Institutes of Health
11th Asia Oceania Human Proteome Organization (AOHUPO) and 7th Asia Oceania Agricultural Proteomics Organization (AOAPO) Congress
08 May 2023 - 10 May 2023
14th Proteomics Conference in Proteins and Proteomics
03 Nov 2022 - 05 Nov 2022
Conference on Implications of Mastitis
19 Oct 2022 - 20 Oct 2022
In-Depth Bovine Milk Analysis to Unearth Goldmine Beneficial for Health Applications
14 Aug 2020 - 14 Aug 2020
College of Veterinary Science and Animal Husbandry
Characterization of Bovine Milk in Health and Disease
29 Aug 2020 - 29 Aug 2020
Lovely Professional University
Purdue University
24 Jun 2022 - 24 Jun 2022
Human Saliva: Body’s Mirror in Health and Disease?
23 Sep 2019 - 23 Sep 2019
Career Symposium 2021 by International Faculty for the Trainees
03 May 2021 - 04 May 2021
National Institutes of Health
The Walk of Life in Biological Laboratory: Dos and Don’ts
12 Mar 2018 - 12 Mar 2018
Indicators of Internal Ecological Factors of Shifting from Bovine Mammary Symbiosis to Dysbiosis: How Early? and How Fast?
29 Jan 2021 - 29 Jan 2021
Proteomics Society of India
The ABC of Grant Writing
18 Apr 2021 - 18 Apr 2021
P Value
Human Saliva: Body’s Mirror in Health and Disease?
20 Mar 2017 - 20 Mar 2017
International Virtual Conference on Emerging Challenges to Veterinary Profession
19 Jun 2021 - 20 Jun 2021
Indian Veterinary Association in Collaboration with Confederation of Indian Industries
Proteomics in Veterinary Research: Bench-to-Pen Side
27 Apr 2019 - 27 Apr 2019
Administrative Positions
Associate Professor
10 Aug 2020 - Present
Indian Institute of Technology Roorkee
Associate Dean of Students' Welfare (Student Wellness)
01 Mar 2020 - 28 Feb 2023
Indian Institute of Technology Roorkee
Faculty Advisor
01 Jan 2018 - 09 Feb 2018
E Summit, Indian Institute of Technology Roorkee
Member Secretary
26 Aug 2020 - Present
Institute Biosafety Committee
Member DRC (Departmental Research Committee
01 Jan 2016 - Present
Dept. of Biotechnology, Indian Institute of Technology Roorkee
Assistant Professor
17 Jan 2014 - 10 Aug 2020
Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Chief Warden
01 Jul 2018 - 30 Jun 2020
Rajiv Bhawan, Indian Institute of Technology Roorkee
01 Jul 2016 - 30 Jun 2018
Rajiv Bhawan, Indian Institute of Technology Roorkee
Staff Advisor Students Club, Cricket
01 Jul 2016 - 30 Jun 2017
Indian Institute of Technology Roorkee
Other work

- Journal Reviewer

1. Food Chemistry, 2. Food Research International, 3. PLoS ONE, 4. Journal of Food Engineering, 4. BMC Veterinary Research, 5. Bioanalytical Chemistry, 6. Cells

Books Authored

Book Chapter

1. Ayushi Kapoor, Monica Yadav, Aparna Verma, Kiran Ambatipudi (2021). Biotechnological Applications in Food Production, Processing, Packaging, and Safety. In: Sustainable Agriculture Reviews 54: Animal Biotechnology for Livestock Production. Editors: Vinod Kumar Yata, Ashok Kumar Mohanty, and Eric Lichtfouse. Publisher: Springer Nature. (In Press)

2. Kuldeep Giri, Vinod Singh Bisht, Sudipa Maity, Kiran Ambatipudi (2020). Biotechnological Advances for Microbiology, Molecular Biology, and Nanotechnology: An Interdisciplinary Approach to the Life Sciences. Editors: Rout, Kerry, and Dutta. Chapter 14, Apple Academic Press. (In Press)

2. Marcelo A. Catalán, Kiran S. Ambatipudi and James E. Melvin. (2012). Salivary Gland Secretion. In: Physiology of the Gastrointestinal Tract. Chapter: 45, Elsevier. Editor: Leonard R. Johnson, Academic Press, 2012, pp. 1229-1250.