Saugata Hazra - Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee
Saugata Hazra
Saugata Hazra Associate professor saugata.hazra@bt.iitr.ac.in +91-1332-284800
Areas of Interest
  • Studying Antimicrobial Drug Resistance, targeting enzyme beta-lactamase & folate biosynthesis pathway, Structural Biology, Protein crystallography, Structure based drug development, Enzymology, Enzyme kinetics, Biophysical & biochemical studies, Structure-function relationship, Computational Biology, MD simulation, Virtual screening, Macromolecular interaction
Professional Background
FromPeriodPositionOrganisation
2011-01-013 years Postdoctoral Research AssociateAlbert Einstein College of Medicine, New York, USA
2010-01-011 year Postdoctoral Research FellowSt Jude Children's Research Hospital, Memphis, USA
Multiple Posts
FromPeriodPositionOrganisation
2020-08-04OngoingAdjunct Faculty, Centre for Nanotechnology, IIT-Roorkee.Indian Institute of Technology, Roorkee
Honors and Awards
AwardInstituteYear
Frontiers of Engineering for DevelopmentRoyal Academy of Engineering, UK2017
Educational Details
DegreeSubjectUniversityYear
Ph.D.Protein Engineering, Structural Biology & Drug DesigningUniversity of Illinois at Chicago2010
M.Tech.Biotechnology and Biochemical EngineeringIIT-Kharagpur2004
M.Sc.Biophysics & Molecular BiologyUniversity of Calcutta2002
B.Sc.Chemistry (Hons.)University of Calcutta2000
Sponsored Research Projects
TopicFunding AgencyStart DatePeriod
Identification and characterization of novel inhibitors against medicinally important beta-lactamaseMHRD2014-016 years 7 months
Development of Novel therapeutics against drug resistant beta-lactamases.SERB, DST2015-01Ongoing
Developing novel antimicrobial therapeutics by exploring multi-enzyme targets.ICMR2017-01Ongoing
Edible oil blend from lignocellulosic pentosans and other inexpensive raw materials.DBT-BIRAC2018-01Ongoing
Combating Carbapenem Resistant Enterobacteriacae (CRE): Using combinatorial approach of conventionalMHRD, Govt. of India2019-01Ongoing
Memberships
  • American Chemical Society (ACS), Member
  • National Network for Mathematical and Computational Biology (NNMCB), Member
  • ADNAT (Association for the promotion of DNA fingerprinting and other DNA technologies), Member
Teaching Engagements
TitleCourse CodeClass NameSemester
BIOINFORMATICSBT-205UGAutumn
MACROMOLECULAR CRYSTALLOGRAPHYIBT-02UGAutumn
BIOINFORMATICSBTN-630PGSpring
MACROMOLECULAR CRYSTALLOGRAPHYIBT-02UGSpring
BIOMOLECULAR MODELLINGBT-460UGAutumn
Nanoscale Modeling and SimulationNTN-504Nanoscale Modeling and SimulationSpring
Computational BiologyBTN-664Computational BiologySpring
Biotechnology Laboratory (II)BTN-304Biotechnology Laboratory (II)Spring
Training SeminarBTN-499Training SeminarAutumn
Research Scholar Groups
Scholar NameInterest
Gazal KalyanMacromolecular Analysis Toolkit (MAT): Taking enzyme beta-lactamase in focus.
Gireesh Kumar ShrotiScreening, isolation and characterization of biodegradable polymer from waste resources
Preeti TomerScreening, isolation and characterization of biodegradable polymer from waste resources
Vivek JunghareDevelopment of database correlation primarily taking enzyme beta-lactamase
Niteesh Kumar PandeyBeta-lactamase inhibitory protein (BLIP): Understanding their interactions with beta-lactamase.
Sourya BhattacharyaUnderstanding Class-A beta lactamase towards drug resistance
Abirlal MukherjeeUnderstanding and characterizing enzyme metallo beta lactamase.
Participation in short term courses
Couse NameSponsored ByDate
Methods and techniques in integrated Structural Biology: Toward structure based drug developmentMHRD Scheme on Global Initiative on Academic Network (GIAN)2020-08
NPTEL course on Structural Biology2021-01
National International Collaboration
TopicOrganisation
In detailed study of food derived peptides and designed peptidomimetics towards therapeuticsUniversity of Nebraska-Lincoln, USA
Development of biodegradable green polymers as 3D printing materials.University College London, UK
Surface fluidics and low cost microscopy for point of care microbial diagonostics in low resource settings.University College London, UK
(A) Exploring unique chemistry of beta-lactam and beta-lactamase for point-of-care microbial diagnostics in low resource setting using surface fluidics and low cost microscopy. (B) Applying theranostics and nanotechnology based drug delivery for new geneUniversity of Cambridge, UK
Edible oil blend from lignocellulosic and other inexpensive raw materialsIIP, Dehradun, India
(A) Unravelling the mechanism for cross talk of signalling pathways involving epithelial mesenchymal transition (EMT) in cancer metastasis. (B) Studying the anticancer activity of dendrimer based nanobot drugs. CSIR-NEIST, Jorhat, Assam, India
Structure-function understanding of proteins involved in biofilm formation.BITS-Pilani, Goa, India
(A) Novel enzyme classification system applying machine learning approach targeting enzyme beta-lactamase. (B)Developing computational tools for studying macromolecules and macromolecular interactions.Nanyang Technological University, Singapore
(A) Metagenomics and PCR-based sequencing approach to identify drug resistant beta-lactmases from farm soil and waste water resources. (B) Studying cattle genome and proteome during environmental thermal stress. (C) Functional genomic and proteomic analICAR - CENTRAL INSTITUTE FOR RESEARCH ON CATTLE (CIRC), Meerut, India
Refereed Journal Papers

1. Sourya Bhattacharya, Aditya K. Padhi, Vivek Junghare, Neeladri singha Das, Debashish Ghosh, Partha Roy, Kam Y.J. Zhang, Saugata Hazra. Understanding the molecular interactions of inhibitors against Bla1 beta-lactamase towards unraveling the mechanism of antimicrobial resistance, IJBM 2021, Just Accepted

2. D Bandekar, S Mohapatra, M Hazra, Saugata Hazra, S Biswas, N-terminal truncation of VC0395_0300 protein from Vibrio cholerae does not lead to loss of diguanylate cyclase activity, Biophysical Chemistry, 106493, 2021

3. A Jana, SK Halder, D Dasgupta, Saugata Hazra, P Mondal, T Bhaskar, D Ghosh. Keratinase Biosynthesis from Waste Poultry Feathers for Proteinaceous Stain Removal, ACS Sustainable Chemistry & Engineering 8 (48), 17651-17663, 2020

4. Yadav, Indu; Purohit, Shivdutt; Singh, Hemant; Bhushan, Sakchi; Yadav, Manoj; Velpandian, Thirumurthy; Chawla, Rohan; Hazra, Saugata; Mishra, Narayan, Vitreous Substitutes: An Overview of the Properties, Importance and Development", Journal of Biomedical Materials Research, Part B - Applied Biomaterials, JBMR-B-20-0466.R1, Just Accepted.

5. D Bandekar, S Mohapatra, M Hazra, Saugata Hazra, S Biswas, N-terminal truncation of VC0395_0300 protein from Vibrio cholerae does not lead to loss of diguanylate cyclase activity, Biophysical Chemistry, 106493, 2020

6. A Das, P Basak, A Pramanik, R Majumder, A Ghosh, Saugata Hazra, M Guria, S. Banik, Ribosylation induced structural changes in Bovine Serum Albumin: understanding high dietary sugar induced protein aggregation and amyloid formation, Heliyon 6 (9), e05053, 2020

7. Megha Sailwal, Amar Jyoti Das, Rajesh K Gazara, Diptarka Dasgupta, Thallada Bhaskar, Saugata Hazra, Debashish Ghosh, Connecting the Dots: Advances in modern metabolomics and its application in yeast system Just Accepted in Biotechnology Advance (JBA).

8. R Deb, GS Sengar, V Junghare, Saugata Hazra, U Singh, R Alex, A Kumar, Characterization of a putative ribosome binding site at the 5′ untranslated region of bovine heat shock protein 90, Molecular Biology Reports 47 (9), 7061-7071, 2020

9. Swati Mohapatra, Nitish Pandey, Saikat Dey, Diptarka Dasgupta, Parsenjit Mondal, Debashish Ghosh, Saugata Hazra. Production of Biodegradable Polymers (PHAs) by Soil Microbes Utilizing Waste Materials as Carbon Source. Frontiers in Soil and Environmental Microbiology, March, 2020, 237-246

10. Tripti Sharma, Diptarka Dasgupta, Preeti Sagar, Arijit Jana, Neeraj Atray, Siddharth S Ray, Debashish Ghosh, Saugata Hazra. Renewable Hydrocarbon from Biomass: Thermo-Chemical, Chemical and Biochemical Perspectives. Frontiers in Soil and Environmental Microbiology, March, 2020, 147-158

11. S Bhattacharya, V Junghare, NK Pandey, D Ghosh, H Patra, Hazra Saugata. An insight into the complete biophysical and biochemical characterization of novel class A beta-lactamase (Bla1) from Bacillus anthracis; International Journal of Biological Macromolecules, 2020, 145, 510-526

12. G Kalyan, V Junghare, S Bhattacharya, Hazra Saugata. Understanding structure-based dynamic interactions of antihypertensive peptides extracted from food sources. Journal of Biomolecular Structure and Dynamics, 2020, 1-15

13. N Bansal, D Dasgupta, Hazra Saugata, T Bhaskar, A Ray, D Ghosh. Effect of utilization of crude glycerol as substrate on fatty acid composition of an oleaginous yeast Rhodotorula mucilagenosa IIPL32: Assessment of Nutritional Indices. Bioresource Technology, April 2020 

14. Ayan Banerjee, Tripti Sharma, Abhilek K Nautiya, Diptarka Dasgupta, Hazra Saugata, Thallada Bhaskar, Debashish Ghosh. Scale-up strategy for yeast single cell oil production for Rhodotorula mucilagenosa IIPL32 from corn cob derived pentosan. Bioresource Technology April 2020 

15. Singh R*, Junghare V*, Hazra Saugata, Singh U, Sengar G, Raja T, Kumar S, Tyagi S, Das A, Kumar A, Koringa P, Jakhesara S, Joshi C, DebR. Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls. Theriogenology; (In Press, Accepted Manuscript). 

16. Sheetal Bandhu, Neha Bansal, Diptarka Dasgupta, Vivek Junghare, Arushdeep Sidana, Gazal Kalyan, Hazra Saugata, Debashish Ghosh. Overproduction of single cell oil from xylose rich sugarcane bagasse hydrolysate by an engineered oleaginous yeast Rhodotorula mucilaginosa IIPL32; 2019, Fuel 254, 115653

17. Omvir Singh, Tripti Sharma, Indrajit Ghosh, Diptarka Dasgupta, Bhanu Prasad Vempatapu, Hazra Saugata, Alexander L Kustov, Bipul Sarkar, Debashish Ghosh. Converting Lignocellulosic Pentosan-Derived Yeast Single Cell Oil into Aromatics: Biomass to Bio-BTX; ACS Sustainable Chemistry & Engineering, 2019, 7 (15), 13437-13445

18. Das V, Bhattacharya S, Chikkaputtaiah C, Pal M#, Hazra Saugata # The basics of epithelial-mesenchymal transition (EMT): A study from a structure, dynamics, and functional perspective. J Cell Physiol. 2019 Feb 5. doi: 10.1002/jcp.28160. 

19. Bhattacharya S, Bhattacharya S, Gachhui R, Hazra Saugata, Mukherjee J. U32 collagenase from Pseudoalteromonas agarivoransNW4327: Activity, structure, substrate interactions and molecular dynamics simulations. IJBM, 2019; 124:635-650.

20. Padhi A, Hazra S. Insights into the role of D-amino acid oxidase mutations in amyotrophic lateral sclerosis. JCB, 2019; 120(2):2180-2197.

21. Wang Wang, Mengcheng Shen, Conrad Fischer, Ratnadeep Basu, Hazra Saugata, Pierre Couvineau, Manish Paul, Faqi Wang, Sandra Toth, Doran S Mix, Marko Poglitsch, Norma P Gerard, Michel Bouvier, John C Vederas, Josef M Penninger, Zamaneh Kassiri, Gavin Y Oudit. Apelin protects against abdominal aortic aneurysm and the therapeutic role of neutral endopeptidase resistant apelin analogs.; 2019, Proceedings of the National Academy of Sciences 116 (26), 13006-13015

22. Dasgupta D, Jhungare V, Nautiyal A, Jana A, Hazra Saugata, Ghosh D. Xylitol production from lignocellulosic pentosans: a rational strain engineering approach towards a multiproduct biorefinery. JAFC,2019, 67 (4), pp 1173–1186.

23. Patel V, Zhabyeyev P, Chen X, Wang F, Paul M, Fan D, McLean B, Basu R, Zhang P, Shah S, Dawson J, Pyle W, Hazra M, Kassiri Z, Hazra Saugata, Vanhaesebroeck B, McCulloch C, Oudit G. PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease. Nature Communications. 9, Article number: 5390 (2018).

24. Sengar G, Deb R, Singh U, Junghare V, Hazra Saugata, Raja T, Alex R, Kumar A, Alyethodi R, Kant R, Jakshara S, Joshi C. Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress. Cell Stress and Chaperones, Available online 18 May 2018.

25. Abhilek Kumar Nautiyal, Arijit Jana, Sourya Bhattacharya, Tripti Sharma, Neha Bansal, Sree Sai Ogetiammini, Debashish Ghosh, Diptarka Dasgupta and Saugata Hazra: Microbial CYP450: An Insight into Its Molecular/Catalytic Mechanism, Production and Industrial Application. Principles and Applications of Fermentation Technology, 03rd Aug, 2018, 189-215

26. Neha Bansal, Mahesh B Khot, Arijit Jana, Abhilek K Nautiyal, Tripti Sharma, Diptarka Dasgupta, Swati Mohapatra, Sanoj Kumar Yadav, Debashish Ghosh and Saugata Hazra: Oleaginous Yeasts: Lignocellulosic Biomass Derived Single Cell Oil as Biofuel Feedstock. 03rd Aug, 2018, 263-306.

27. Pal M, Bhattacharya S, Kalyan G, Hazra Saugata. Cadherin profiling for therapeutic interventions in Epithelial Mesenchymal Transition (EMT) and tumorigenesis. Experimental Cell Research, Available online 16 April 2018.

28. Singh R, Sengar G, Singh U, Deb R, Junghare V, Hazra Saugata, Kumar S, Tyagi S, Das A, Raja T, Kumar A.Functional proteomic analysis of crossbred (Holstein Friesian × Sahiwal) bull spermatozoa. Reproduction in Domestic Animals. 2018.

29. Bandhu S, Khot M, Sharma T, Sharma O, Dasgupta D, Mohapatra S, Hazra Saugata, Khatri O, Ghosh D. Single Cell Oil from Oleaginous Yeast Grown on Sugarcane Bagasse-Derived Xylose: An Approach toward Novel Biolubricant for Low Friction and Wear. ACS Sustainable Chemistry & Engineering. 2017;6(1):275-283.

30. Bandekar D, Chouhan O, Mohapatra S, Hazra M, Hazra Saugata, Biswas S. Putative protein VC0395_0300 from Vibrio cholerae is a diguanylate cyclase with a role in biofilm formation. Microbiological Research. 2017; 202:61-70.

31. Das V, Kalyan G, Hazra Saugata, Pal M. Understanding the role of structural integrity and differential expression of integrin profiling to identify potential therapeutic targets in breast cancer. Journal of Cellular Physiology. 2017;233(1):168-185.

32. Bharatiy S, Hazra M, Paul M, Mohapatra S, Samantaray D, Dubey R, Sanyal S, Datta S, Hazra Saugata. In Silico Designing of an Industrially Sustainable Carbonic Anhydrase Using Molecular Dynamics Simulation. ACS Omega. 2016;1(6):1081-1103.

33. Wang W, McKinnie S, Farhan M, Paul M, McDonald T, McLean B, Llorens-Cortes C, Hazra Saugata, Murray A, Vederas J, Oudit G. Angiotensin-Converting Enzyme 2 Metabolizes and Partially Inactivates Pyr-Apelin-13 and Apelin-17Novelty and Significance. Hypertension. 2016;68(2):365-377.

34. Chouhan O, Bandekar D, Hazra M, Baghudana A, Hazra Saugata, Biswas S. Effect of site-directed mutagenesis at the GGEEF domain of the biofilm forming GGEEF protein from Vibrio cholerae. AMB Express. 2016;6(1).

35. Hazra Saugata, Kurz S, Wolff K, Nguyen L, Bonomo R, Blanchard J. Kinetic and Structural Characterization of the Interaction of 6-Methylidene Penem 2 with the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2015;54(36):5657-5664.

36. Kurz S*, Hazra Saugata*, Bethel C, Romagnoli C, Caselli E, Prati F, Blanchard J, Bonomo R. Inhibiting the β-Lactamase of Mycobacterium tuberculosis (Mtb) with Novel Boronic Acid Transition-State Inhibitors (BATSIs). ACS Infectious Diseases. 2015;1(6):234-242. (* contributed equally)

37. Basu R, Hazra Saugata, Shanks M, Paterson D, Oudit G. Novel Mutation in Exon 14 of the Sarcomere Gene MYH7 in Familial Left Ventricular Noncompaction With Bicuspid Aortic Valve. Circulation: Heart Failure. 2014;7(6):1059-1062.

38. Hazra Saugata, Xu H, Blanchard J. Tebipenem, a New Carbapenem Antibiotic, Is a Slow Substrate That Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2014;53(22):3671-3678.

39. Hadi T*, Hazra Saugata*, Tanner M, Blanchard J. Structure of MurNAc 6-Phosphate Hydrolase (MurQ) from Haemophilus influenzae with a Bound Inhibitor. Biochemistry. 2013;52(51):9358-9366. (* contributed equally)

40. Kurz S, Wolff K, Hazra Saugata, Bethel C, Hujer A, Smith K et al. Can Inhibitor-Resistant Substitutions in the Mycobacterium tuberculosis β-Lactamase BlaC Lead to Clavulanate Resistance?: a Biochemical Rationale for the Use of β-Lactam–β-Lactamase Inhibitor Combinations. AAC. 2013;57(12):6085-6096.

41. Paul M, Hazra M, Barman A, Hazra Saugata. Comparative molecular dynamics simulation studies for determining factors contributing to the thermostability of chemotaxis protein “CheY”. JBSD. 2013;32(6):928-949.

42. Quartararo C, Hazra Saugata, Hadi T, Blanchard J. Structural, Kinetic and Chemical Mechanism of Isocitrate Dehydrogenase-1 from Mycobacterium tuberculosis. Biochemistry. 2013;52(10):1765-1775.

43. Xu H, Hazra Saugata, Blanchard J. NXL104 Irreversibly Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2012;51(22):4551-4557.

44. Hazra Saugata, Szewczak A, Ort S, Konrad M, Lavie A. Post-Translational Phosphorylation of Serine 74 of Human Deoxycytidine Kinase Favors the Enzyme Adopting the Open Conformation Making It Competent for Nucleoside Binding and Release. Biochemistry. 2011;50(14):2870-2880.

45. Hazra Saugata, Konrad M, Lavie A. The Sugar Ring of the Nucleoside Is Required for Productive Substrate Positioning in the Active Site of Human Deoxycytidine Kinase (dCK): Implications for the Development of dCK-Activated Acyclic Guanine Analogues. JMC. 2010;53(15):5792-5800.

46. Hazra Saugata, Ort S, Konrad M, Lavie A. Structural and Kinetic Characterization of Human Deoxycytidine Kinase Variants Able To Phosphorylate 5-Substituted Deoxycytidine and Thymidine Analogues. Biochemistry. 2010;49(31):6784-6790.

47. Hazra Saugata, Sabini E, Ort S, Konrad M, Lavie A. Extending Thymidine Kinase Activity to the Catalytic Repertoire of Human Deoxycytidine Kinase. Biochemistry. 2009;48(6):1256-1263.

48. Sabini E, Hazra Saugata, Konrad M, Lavie A. Elucidation of Different Binding Modes of Purine Nucleosides to Human Deoxycytidine Kinase. JMC. 2008;51(14):4219-4225.

49. Sabini E, Hazra Saugata, Ort S, Konrad M, Lavie A. Structural Basis for Substrate Promiscuity of dCK. JMB,2008;378(3):607-621.

50. McSorley T, Ort S, Hazra Saugata, Lavie A, Konrad M. Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues. FEBS Letters. 2008;582(5):720-724.

51. Sabini E, Hazra Saugata, Konrad M, Lavie A. Nonenantioselectivity Property of Human Deoxycytidine Kinase Explained by Structures of the Enzyme in Complex with L- and D-Nucleosides. JMC. 2007;50(13):3004-3014.

52. Sabini E, Hazra Saugata, Konrad M, Burley S, Lavie A. Structural basis for activation of the therapeutic L-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase. NAR. 2006;35(1):186-192.

 

Books Authored

Book Chapters:

  1. Microbial CYP450: An Insight into Its Molecular/Catalytic Mechanism, Production and Industrial Application. (2018).
  2. Oleaginous Yeasts: Lignocellulosic Biomass Derived Single Cell Oil as Biofuel Feedstock. (2018).
Patents

Detail of patents:

Patent1: Identification of functional internal ribosomal entry sites (IRES) at bovine heat shock protein 90 (Application Number: 201711042562)

Patent2: A METHOD OF PRODUCING THE POLYHYDROXYALKANOATE (PHA) USING WASTE WATER: (Application No.  202011031817)

 

Patent3: BL-Tester: An easy, cheap and reliable culture free point-of-care (ERC-PoC) diagnosis to detect antimicrobial resistance (Application No. 202111003064)

 

 

Databases & Web server

Hazra group is constantly working on development of programs which would facilitates biologist without computational expertise to use those tools to analyze and process big data. Following are few of our servers

Antihypertensive Peptide Database:

http://hazralab.iitr.ac.in/ahdb/index.php

AHPP:

http://hazralab.iitr.ac.in/ahtpg.php

AMPGP:

http://hazralab.iitr.ac.in/ampgp.php

mi-RNA Database:

http://hazralab.iitr.ac.in/dmircattle/index.php

MAT:

mat.iitr.ac.in

 

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