Saugata Hazra
Areas of Interest
- Studying Antimicrobial Drug Resistance, targeting enzyme beta-lactamase & folate biosynthesis pathway
- Structural Biology, Protein crystallography, Structure based drug development
- Enzymology, Enzyme kinetics, Biophysical & biochemical studies, Structure-function relationship
- Computational Biology, MD simulation, Virtual screening, Macromolecular interaction
Professional Background
| From | To | Designation | Organisation |
|---|---|---|---|
| 2011 | 2014 | Postdoctoral Research Associate | Albert Einstein College of Medicine, New York, USA |
| 2010 | 2011 | Postdoctoral Research Fellow | St Jude Children's Research Hospital, Memphis, USA |
Honors and Awards
| Award | Institute | Year |
|---|---|---|
| Frontiers of Engineering for Development | Royal Academy of Engineering, UK | 2017 |
Educational Details
| Degree | Subject | University | Year |
|---|---|---|---|
| Ph.D. | Protein Engineering, Structural Biology & Drug Designing | University of Illinois at Chicago | 2010 |
| M.Tech. | Biotechnology and Biochemical Engineering | IIT-Kharagpur | 2004 |
| M.Sc. | Biophysics & Molecular Biology | University of Calcutta | 2002 |
| B.Sc. | Chemistry (Hons.) | University of Calcutta | 2000 |
Sponsored Research Projects
| Topic | Funding Agency | Year |
|---|---|---|
| Identification and characterization of novel inhibitors against medicinally important beta-lactamase | MHRD | 2014 |
| Development of Novel therapeutics against drug resistant beta-lactamases. | SERB, DST | 2015 |
| Developing novel antimicrobial therapeutics by exploring multi-enzyme targets. | ICMR | 2017 |
| Edible oil blend from lignocellulosic pentosans and other inexpensive raw materials. | DBT-BIRAC | 2018 |
| Combating Carbapenem Resistant Enterobacteriacae (CRE): Using combinatorial approach of conventional | MHRD, Govt. of India | 2019 |
Memberships
- American Chemical Society (ACS), Member
- National Network for Mathematical and Computational Biology (NNMCB), Member
- ADNAT (Association for the promotion of DNA fingerprinting and other DNA technologies), Member
Teaching Engagements
| Title | Course Code | Class Name | Semester |
|---|---|---|---|
| BIOINFORMATICS | BT-205 | UG | Autumn |
| MACROMOLECULAR CRYSTALLOGRAPHY | IBT-02 | UG | Autumn |
| BIOINFORMATICS | BTN-630 | PG | Spring |
| MACROMOLECULAR CRYSTALLOGRAPHY | IBT-02 | UG | Spring |
| BIOMOLECULAR MODELLING | BT-460 | UG | Autumn |
| Nanoscale Modeling and Simulation | NTN-504 | Nanoscale Modeling and Simulation | Spring |
| Computational Biology | BTN-664 | Computational Biology | Spring |
| Biotechnology Laboratory (II) | BTN-304 | Biotechnology Laboratory (II) | Spring |
| Training Seminar | BTN-499 | Training Seminar | Autumn |
Research Scholar Groups
| Scholar Name | Interest |
|---|---|
| Gazal Kalyan | Macromolecular Analysis Toolkit (MAT): Taking enzyme beta-lactamase in focus. |
| Gireesh Kumar Shroti | Screening, isolation and characterization of biodegradable polymer from waste resources |
| Preeti Tomer | Screening, isolation and characterization of biodegradable polymer from waste resources |
| Vivek Junghare | Development of database correlation primarily taking enzyme beta-lactamase |
| Niteesh Kumar Pandey | Beta-lactamase inhibitory protein (BLIP): Understanding their interactions with beta-lactamase. |
| Sourya Bhattacharya | Understanding Class-A beta lactamase towards drug resistance |
| Abirlal Mukherjee | Understanding and characterizing enzyme metallo beta lactamase. |
Participation in short term courses
| Couse Name | Sponsored By | Date |
|---|---|---|
| Methods and techniques in integrated Structural Biology: Toward structure based drug development | MHRD Scheme on Global Initiative on Academic Network (GIAN) | 15.01.2018 |
National International Collaboration
| Topic | Organisation | Level |
|---|---|---|
| In detailed study of food derived peptides and designed peptidomimetics towards therapeutics | University of Nebraska-Lincoln, USA | RP |
| Development of biodegradable green polymers as 3D printing materials. | University College London, UK | RP |
| Surface fluidics and low cost microscopy for point of care microbial diagonostics in low resource settings. | University College London, UK | RP |
| (A) Exploring unique chemistry of beta-lactam and beta-lactamase for point-of-care microbial diagnostics in low resource setting using surface fluidics and low cost microscopy. (B) Applying theranostics and nanotechnology based drug delivery for new generation beta-lactamase therapeutics. | University of Cambridge, UK | RP |
| Edible oil blend from lignocellulosic and other inexpensive raw materials | IIP, Dehradun, India | RP |
| (A) Unravelling the mechanism for cross talk of signalling pathways involving epithelial mesenchymal transition (EMT) in cancer metastasis. (B) Studying the anticancer activity of dendrimer based nanobot drugs. | CSIR-NEIST, Jorhat, Assam, India | RP |
| Structure-function understanding of proteins involved in biofilm formation. | BITS-Pilani, Goa, India | RP |
| (A) Novel enzyme classification system applying machine learning approach targeting enzyme beta-lactamase. (B)Developing computational tools for studying macromolecules and macromolecular interactions. | Nanyang Technological University, Singapore | RP |
| (A) Metagenomics and PCR-based sequencing approach to identify drug resistant beta-lactmases from farm soil and waste water resources. (B) Studying cattle genome and proteome during environmental thermal stress. (C) Functional genomic and proteomic analysis of cross breed bull spermatozoa. | ICAR - CENTRAL INSTITUTE FOR RESEARCH ON CATTLE (CIRC), Meerut, India | RP |
Books Authored
Book Chapters:
- Microbial CYP450: An Insight into Its Molecular/Catalytic Mechanism, Production and Industrial Application. (2018).
- Oleaginous Yeasts: Lignocellulosic Biomass Derived Single Cell Oil as Biofuel Feedstock. (2018).
Refereed Journal Papers
- Singh R*, Junghare V*, Hazra S, Singh U, Sengar G, Raja T, Kumar S, Tyagi S, Das A, Kumar A, Koringa P, Jakhesara S, Joshi C, Deb R. Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls. Theriogenology; Available online 14 February 2019 (In Press, Accepted Manuscript). (* contributed equally)
- Das V, Bhattacharya S, Chikkaputtaiah C, Hazra S#, Pal M#. The basics of epithelial-mesenchymal transition (EMT): A study from a structure, dynamics, and functional perspective. J Cell Physiol. 2019 Feb 5. doi: 10.1002/jcp.28160. [Epub ahead of print] (# corresponding author)
- Bhattacharya S, Bhattacharya S, Gachhui R, Hazra S, Mukherjee J. U32 collagenase from Pseudoalteromonas agarivoransNW4327: Activity, structure, substrate interactions and molecular dynamics simulations. International Journal of Biological Macromolecules, 2019; 124:635-650.
- Padhi A, Hazra S. Insights into the role of D-amino acid oxidase mutations in amyotrophic lateral sclerosis. Journal of Cellular Biochemistry, 2019; 120(2):2180-2197.
- Dasgupta D, Jhungare V, Nautiyal A, Jana A, Hazra S, Ghosh D. Xylitol production from lignocellulosic pentosans: a rational strain engineering approach towards a multiproduct biorefinery. J. Agric. Food Chem, 2019, 67 (4), pp 1173–1186.
- Patel V, Zhabyeyev P, Chen X, Wang F, Paul M, Fan D, McLean B, Basu R, Zhang P, Shah S, Dawson J, Pyle W, Hazra M, Kassiri Z, Hazra S, Vanhaesebroeck B, McCulloch C, Oudit G. PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease. Nature Communications. 9, Article number: 5390 (2018).
- Sengar G, Deb R, Singh U, Junghare V, Hazra S, Raja T, Alex R, Kumar A, Alyethodi R, Kant R, Jakshara S, Joshi C. Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress. Cell Stress and Chaperones, Available online 18 May 2018.
- Pal M, Bhattacharya S, Kalyan G, Hazra S. Cadherin profiling for therapeutic interventions in Epithelial Mesenchymal Transition (EMT) and tumorigenesis. Experimental Cell Research, Available online 16 April 2018.
- Singh R, Sengar G, Singh U, Deb R, Junghare V, Hazra S, Kumar S, Tyagi S, Das A, Raja T, Kumar A. Functional proteomic analysis of crossbred (Holstein Friesian × Sahiwal) bull spermatozoa. Reproduction in Domestic Animals. 2018.
- Bandhu S, Khot M, Sharma T, Sharma O, Dasgupta D, Mohapatra S, Hazra S, Khatri O, Ghosh D. Single Cell Oil from Oleaginous Yeast Grown on Sugarcane Bagasse-Derived Xylose: An Approach toward Novel Biolubricant for Low Friction and Wear. ACS Sustainable Chemistry & Engineering. 2017;6(1):275-283.
- Bandekar D, Chouhan O, Mohapatra S, Hazra M, Hazra S, Biswas S. Putative protein VC0395_0300 from Vibrio cholerae is a diguanylate cyclase with a role in biofilm formation. Microbiological Research. 2017;202:61-70.
- Das V, Kalyan G, Hazra S, Pal M. Understanding the role of structural integrity and differential expression of integrin profiling to identify potential therapeutic targets in breast cancer. Journal of Cellular Physiology. 2017;233(1):168-185.
- Bharatiy S, Hazra M, Paul M, Mohapatra S, Samantaray D, Dubey R, Sanyal S, Datta S, Hazra S. In Silico Designing of an Industrially Sustainable Carbonic Anhydrase Using Molecular Dynamics Simulation. ACS Omega. 2016;1(6):1081-1103.
- Wang W, McKinnie S, Farhan M, Paul M, McDonald T, McLean B, Llorens-Cortes C, Hazra S, Murray A, Vederas J, Oudit G. Angiotensin-Converting Enzyme 2 Metabolizes and Partially Inactivates Pyr-Apelin-13 and Apelin-17Novelty and Significance. Hypertension. 2016;68(2):365-377.
- Chouhan O, Bandekar D, Hazra M, Baghudana A, Hazra S, Biswas S. Effect of site-directed mutagenesis at the GGEEF domain of the biofilm forming GGEEF protein from Vibrio cholerae. AMB Express. 2016;6(1).
- Hazra S, Kurz S, Wolff K, Nguyen L, Bonomo R, Blanchard J. Kinetic and Structural Characterization of the Interaction of 6-Methylidene Penem 2 with the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2015;54(36):5657-5664.
- Kurz S*, Hazra S*, Bethel C, Romagnoli C, Caselli E, Prati F, Blanchard J, Bonomo R. Inhibiting the β-Lactamase of Mycobacterium tuberculosis (Mtb) with Novel Boronic Acid Transition-State Inhibitors (BATSIs). ACS Infectious Diseases. 2015;1(6):234-242. (* contributed equally)
- Basu R, Hazra S, Shanks M, Paterson D, Oudit G. Novel Mutation in Exon 14 of the Sarcomere Gene MYH7 in Familial Left Ventricular Noncompaction With Bicuspid Aortic Valve. Circulation: Heart Failure. 2014;7(6):1059-1062.
- Hazra S, Xu H, Blanchard J. Tebipenem, a New Carbapenem Antibiotic, Is a Slow Substrate That Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2014;53(22):3671-3678.
- Hadi T*, Hazra S*, Tanner M, Blanchard J. Structure of MurNAc 6-Phosphate Hydrolase (MurQ) from Haemophilus influenzae with a Bound Inhibitor. Biochemistry. 2013;52(51):9358-9366. (* contributed equally)
- Kurz S, Wolff K, Hazra S, Bethel C, Hujer A, Smith K et al. Can Inhibitor-Resistant Substitutions in the Mycobacterium tuberculosis β-Lactamase BlaC Lead to Clavulanate Resistance?: a Biochemical Rationale for the Use of β-Lactam–β-Lactamase Inhibitor Combinations. Antimicrobial Agents and Chemotherapy. 2013;57(12):6085-6096.
- Paul M, Hazra M, Barman A, Hazra S. Comparative molecular dynamics simulation studies for determining factors contributing to the thermostability of chemotaxis protein “CheY”. Journal of Biomolecular Structure and Dynamics. 2013;32(6):928-949.
- Quartararo C, Hazra S, Hadi T, Blanchard J. Structural, Kinetic and Chemical Mechanism of Isocitrate Dehydrogenase-1 from Mycobacterium tuberculosis. Biochemistry. 2013;52(10):1765-1775.
- Xu H, Hazra S, Blanchard J. NXL104 Irreversibly Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2012;51(22):4551-4557.
- Hazra S, Szewczak A, Ort S, Konrad M, Lavie A. Post-Translational Phosphorylation of Serine 74 of Human Deoxycytidine Kinase Favors the Enzyme Adopting the Open Conformation Making It Competent for Nucleoside Binding and Release. Biochemistry. 2011;50(14):2870-2880.
- Hazra S, Konrad M, Lavie A. The Sugar Ring of the Nucleoside Is Required for Productive Substrate Positioning in the Active Site of Human Deoxycytidine Kinase (dCK): Implications for the Development of dCK-Activated Acyclic Guanine Analogues. Journal of Medicinal Chemistry. 2010;53(15):5792-5800.
- Hazra S, Ort S, Konrad M, Lavie A. Structural and Kinetic Characterization of Human Deoxycytidine Kinase Variants Able To Phosphorylate 5-Substituted Deoxycytidine and Thymidine Analogues. Biochemistry. 2010;49(31):6784-6790.
- Hazra S, Sabini E, Ort S, Konrad M, Lavie A. Extending Thymidine Kinase Activity to the Catalytic Repertoire of Human Deoxycytidine Kinase. Biochemistry. 2009;48(6):1256-1263.
- Sabini E, Hazra S, Konrad M, Lavie A. Elucidation of Different Binding Modes of Purine Nucleosides to Human Deoxycytidine Kinase. Journal of Medicinal Chemistry. 2008;51(14):4219-4225.
- Sabini E, Hazra S, Ort S, Konrad M, Lavie A. Structural Basis for Substrate Promiscuity of dCK. Journal of Molecular Biology. 2008;378(3):607-621.
- McSorley T, Ort S, Hazra S, Lavie A, Konrad M. Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues. FEBS Letters. 2008;582(5):720-724.
- Sabini E, Hazra S, Konrad M, Lavie A. Nonenantioselectivity Property of Human Deoxycytidine Kinase Explained by Structures of the Enzyme in Complex with L- and D-Nucleosides. Journal of Medicinal Chemistry. 2007;50(13):3004-3014.
- Sabini E, Hazra S, Konrad M, Burley S, Lavie A. Structural basis for activation of the therapeutic L-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase. Nucleic Acids Research. 2006;35(1):186-192.
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