Areas of Interest
- Studying Antimicrobial Drug Resistance, targeting enzyme beta-lactamase & folate biosynthesis pathway, Structural Biology, Protein crystallography, Structure based drug development, Enzymology, Enzyme kinetics, Biophysical & biochemical studies, Structure-function relationship, Computational Biology, MD simulation, Virtual screening, Macromolecular interaction
Professional Background
| From | Period | Position | Organisation |
|---|---|---|---|
| 2011-01-01 | 3 years | Postdoctoral Research Associate | Albert Einstein College of Medicine, New York, USA |
| 2010-01-01 | 1 year | Postdoctoral Research Fellow | St Jude Children's Research Hospital, Memphis, USA |
Multiple Posts
| From | Period | Position | Organisation |
|---|---|---|---|
| 2020-08-04 | Ongoing | Adjunct Faculty, Centre for Nanotechnology, IIT-Roorkee. | Indian Institute of Technology, Roorkee |
Honors and Awards
| Award | Institute | Year |
|---|---|---|
| Frontiers of Engineering for Development | Royal Academy of Engineering, UK | 2017 |
Educational Details
| Degree | Subject | University | Year |
|---|---|---|---|
| Ph.D. | Protein Engineering, Structural Biology & Drug Designing | University of Illinois at Chicago | 2010 |
| M.Tech. | Biotechnology and Biochemical Engineering | IIT-Kharagpur | 2004 |
| M.Sc. | Biophysics & Molecular Biology | University of Calcutta | 2002 |
| B.Sc. | Chemistry (Hons.) | University of Calcutta | 2000 |
Sponsored Research Projects
| Topic | Funding Agency | Start Date | Period |
|---|---|---|---|
| Identification and characterization of novel inhibitors against medicinally important beta-lactamase | MHRD | 2014-01 | 6 years 7 months |
| Development of Novel therapeutics against drug resistant beta-lactamases. | SERB, DST | 2015-01 | Ongoing |
| Developing novel antimicrobial therapeutics by exploring multi-enzyme targets. | ICMR | 2017-01 | Ongoing |
| Edible oil blend from lignocellulosic pentosans and other inexpensive raw materials. | DBT-BIRAC | 2018-01 | Ongoing |
| Combating Carbapenem Resistant Enterobacteriacae (CRE): Using combinatorial approach of conventional | MHRD, Govt. of India | 2019-01 | Ongoing |
Memberships
- American Chemical Society (ACS), Member
- National Network for Mathematical and Computational Biology (NNMCB), Member
- ADNAT (Association for the promotion of DNA fingerprinting and other DNA technologies), Member
Teaching Engagements
| Title | Course Code | Class Name | Semester |
|---|---|---|---|
| BIOINFORMATICS | BT-205 | UG | Autumn |
| MACROMOLECULAR CRYSTALLOGRAPHY | IBT-02 | UG | Autumn |
| BIOINFORMATICS | BTN-630 | PG | Spring |
| MACROMOLECULAR CRYSTALLOGRAPHY | IBT-02 | UG | Spring |
| BIOMOLECULAR MODELLING | BT-460 | UG | Autumn |
| Nanoscale Modeling and Simulation | NTN-504 | Nanoscale Modeling and Simulation | Spring |
| Computational Biology | BTN-664 | Computational Biology | Spring |
| Biotechnology Laboratory (II) | BTN-304 | Biotechnology Laboratory (II) | Spring |
| Training Seminar | BTN-499 | Training Seminar | Autumn |
Research Scholar Groups
| Scholar Name | Interest |
|---|---|
| Gazal Kalyan | Macromolecular Analysis Toolkit (MAT): Taking enzyme beta-lactamase in focus. |
| Gireesh Kumar Shroti | Screening, isolation and characterization of biodegradable polymer from waste resources |
| Preeti Tomer | Screening, isolation and characterization of biodegradable polymer from waste resources |
| Vivek Junghare | Development of database correlation primarily taking enzyme beta-lactamase |
| Niteesh Kumar Pandey | Beta-lactamase inhibitory protein (BLIP): Understanding their interactions with beta-lactamase. |
| Sourya Bhattacharya | Understanding Class-A beta lactamase towards drug resistance |
| Abirlal Mukherjee | Understanding and characterizing enzyme metallo beta lactamase. |
Participation in short term courses
| Couse Name | Sponsored By | Date |
|---|---|---|
| Methods and techniques in integrated Structural Biology: Toward structure based drug development | MHRD Scheme on Global Initiative on Academic Network (GIAN) | 2020-08 |
| NPTEL course on Structural Biology | 2021-01 |
National International Collaboration
| Topic | Organisation |
|---|---|
| In detailed study of food derived peptides and designed peptidomimetics towards therapeutics | University of Nebraska-Lincoln, USA |
| Development of biodegradable green polymers as 3D printing materials. | University College London, UK |
| Surface fluidics and low cost microscopy for point of care microbial diagonostics in low resource settings. | University College London, UK |
| (A) Exploring unique chemistry of beta-lactam and beta-lactamase for point-of-care microbial diagnostics in low resource setting using surface fluidics and low cost microscopy. (B) Applying theranostics and nanotechnology based drug delivery for new gene | University of Cambridge, UK |
| Edible oil blend from lignocellulosic and other inexpensive raw materials | IIP, Dehradun, India |
| (A) Unravelling the mechanism for cross talk of signalling pathways involving epithelial mesenchymal transition (EMT) in cancer metastasis. (B) Studying the anticancer activity of dendrimer based nanobot drugs. | CSIR-NEIST, Jorhat, Assam, India |
| Structure-function understanding of proteins involved in biofilm formation. | BITS-Pilani, Goa, India |
| (A) Novel enzyme classification system applying machine learning approach targeting enzyme beta-lactamase. (B)Developing computational tools for studying macromolecules and macromolecular interactions. | Nanyang Technological University, Singapore |
| (A) Metagenomics and PCR-based sequencing approach to identify drug resistant beta-lactmases from farm soil and waste water resources. (B) Studying cattle genome and proteome during environmental thermal stress. (C) Functional genomic and proteomic anal | ICAR - CENTRAL INSTITUTE FOR RESEARCH ON CATTLE (CIRC), Meerut, India |
Refereed Journal Papers
1. Sourya Bhattacharya, Aditya K. Padhi, Vivek Junghare, Neeladri singha Das, Debashish Ghosh, Partha Roy, Kam Y.J. Zhang, Saugata Hazra. Understanding the molecular interactions of inhibitors against Bla1 beta-lactamase towards unraveling the mechanism of antimicrobial resistance, IJBM 2021, Just Accepted
2. D Bandekar, S Mohapatra, M Hazra, Saugata Hazra, S Biswas, N-terminal truncation of VC0395_0300 protein from Vibrio cholerae does not lead to loss of diguanylate cyclase activity, Biophysical Chemistry, 106493, 2021
3. A Jana, SK Halder, D Dasgupta, Saugata Hazra, P Mondal, T Bhaskar, D Ghosh. Keratinase Biosynthesis from Waste Poultry Feathers for Proteinaceous Stain Removal, ACS Sustainable Chemistry & Engineering 8 (48), 17651-17663, 2020
4. Yadav, Indu; Purohit, Shivdutt; Singh, Hemant; Bhushan, Sakchi; Yadav, Manoj; Velpandian, Thirumurthy; Chawla, Rohan; Hazra, Saugata; Mishra, Narayan, Vitreous Substitutes: An Overview of the Properties, Importance and Development", Journal of Biomedical Materials Research, Part B - Applied Biomaterials, JBMR-B-20-0466.R1, Just Accepted.
5. D Bandekar, S Mohapatra, M Hazra, Saugata Hazra, S Biswas, N-terminal truncation of VC0395_0300 protein from Vibrio cholerae does not lead to loss of diguanylate cyclase activity, Biophysical Chemistry, 106493, 2020
6. A Das, P Basak, A Pramanik, R Majumder, A Ghosh, Saugata Hazra, M Guria, S. Banik, Ribosylation induced structural changes in Bovine Serum Albumin: understanding high dietary sugar induced protein aggregation and amyloid formation, Heliyon 6 (9), e05053, 2020
7. Megha Sailwal, Amar Jyoti Das, Rajesh K Gazara, Diptarka Dasgupta, Thallada Bhaskar, Saugata Hazra, Debashish Ghosh, Connecting the Dots: Advances in modern metabolomics and its application in yeast system Just Accepted in Biotechnology Advance (JBA).
8. R Deb, GS Sengar, V Junghare, Saugata Hazra, U Singh, R Alex, A Kumar, Characterization of a putative ribosome binding site at the 5′ untranslated region of bovine heat shock protein 90, Molecular Biology Reports 47 (9), 7061-7071, 2020
9. Swati Mohapatra, Nitish Pandey, Saikat Dey, Diptarka Dasgupta, Parsenjit Mondal, Debashish Ghosh, Saugata Hazra. Production of Biodegradable Polymers (PHAs) by Soil Microbes Utilizing Waste Materials as Carbon Source. Frontiers in Soil and Environmental Microbiology, March, 2020, 237-246
10. Tripti Sharma, Diptarka Dasgupta, Preeti Sagar, Arijit Jana, Neeraj Atray, Siddharth S Ray, Debashish Ghosh, Saugata Hazra. Renewable Hydrocarbon from Biomass: Thermo-Chemical, Chemical and Biochemical Perspectives. Frontiers in Soil and Environmental Microbiology, March, 2020, 147-158
11. S Bhattacharya, V Junghare, NK Pandey, D Ghosh, H Patra, Hazra Saugata. An insight into the complete biophysical and biochemical characterization of novel class A beta-lactamase (Bla1) from Bacillus anthracis; International Journal of Biological Macromolecules, 2020, 145, 510-526
12. G Kalyan, V Junghare, S Bhattacharya, Hazra Saugata. Understanding structure-based dynamic interactions of antihypertensive peptides extracted from food sources. Journal of Biomolecular Structure and Dynamics, 2020, 1-15
13. N Bansal, D Dasgupta, Hazra Saugata, T Bhaskar, A Ray, D Ghosh. Effect of utilization of crude glycerol as substrate on fatty acid composition of an oleaginous yeast Rhodotorula mucilagenosa IIPL32: Assessment of Nutritional Indices. Bioresource Technology, April 2020
14. Ayan Banerjee, Tripti Sharma, Abhilek K Nautiya, Diptarka Dasgupta, Hazra Saugata, Thallada Bhaskar, Debashish Ghosh. Scale-up strategy for yeast single cell oil production for Rhodotorula mucilagenosa IIPL32 from corn cob derived pentosan. Bioresource Technology April 2020
15. Singh R*, Junghare V*, Hazra Saugata, Singh U, Sengar G, Raja T, Kumar S, Tyagi S, Das A, Kumar A, Koringa P, Jakhesara S, Joshi C, DebR. Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls. Theriogenology; (In Press, Accepted Manuscript).
16. Sheetal Bandhu, Neha Bansal, Diptarka Dasgupta, Vivek Junghare, Arushdeep Sidana, Gazal Kalyan, Hazra Saugata, Debashish Ghosh. Overproduction of single cell oil from xylose rich sugarcane bagasse hydrolysate by an engineered oleaginous yeast Rhodotorula mucilaginosa IIPL32; 2019, Fuel 254, 115653
17. Omvir Singh, Tripti Sharma, Indrajit Ghosh, Diptarka Dasgupta, Bhanu Prasad Vempatapu, Hazra Saugata, Alexander L Kustov, Bipul Sarkar, Debashish Ghosh. Converting Lignocellulosic Pentosan-Derived Yeast Single Cell Oil into Aromatics: Biomass to Bio-BTX; ACS Sustainable Chemistry & Engineering, 2019, 7 (15), 13437-13445
18. Das V, Bhattacharya S, Chikkaputtaiah C, Pal M#, Hazra Saugata # The basics of epithelial-mesenchymal transition (EMT): A study from a structure, dynamics, and functional perspective. J Cell Physiol. 2019 Feb 5. doi: 10.1002/jcp.28160.
19. Bhattacharya S, Bhattacharya S, Gachhui R, Hazra Saugata, Mukherjee J. U32 collagenase from Pseudoalteromonas agarivoransNW4327: Activity, structure, substrate interactions and molecular dynamics simulations. IJBM, 2019; 124:635-650.
20. Padhi A, Hazra S. Insights into the role of D-amino acid oxidase mutations in amyotrophic lateral sclerosis. JCB, 2019; 120(2):2180-2197.
21. Wang Wang, Mengcheng Shen, Conrad Fischer, Ratnadeep Basu, Hazra Saugata, Pierre Couvineau, Manish Paul, Faqi Wang, Sandra Toth, Doran S Mix, Marko Poglitsch, Norma P Gerard, Michel Bouvier, John C Vederas, Josef M Penninger, Zamaneh Kassiri, Gavin Y Oudit. Apelin protects against abdominal aortic aneurysm and the therapeutic role of neutral endopeptidase resistant apelin analogs.; 2019, Proceedings of the National Academy of Sciences 116 (26), 13006-13015
22. Dasgupta D, Jhungare V, Nautiyal A, Jana A, Hazra Saugata, Ghosh D. Xylitol production from lignocellulosic pentosans: a rational strain engineering approach towards a multiproduct biorefinery. JAFC,2019, 67 (4), pp 1173–1186.
23. Patel V, Zhabyeyev P, Chen X, Wang F, Paul M, Fan D, McLean B, Basu R, Zhang P, Shah S, Dawson J, Pyle W, Hazra M, Kassiri Z, Hazra Saugata, Vanhaesebroeck B, McCulloch C, Oudit G. PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease. Nature Communications. 9, Article number: 5390 (2018).
24. Sengar G, Deb R, Singh U, Junghare V, Hazra Saugata, Raja T, Alex R, Kumar A, Alyethodi R, Kant R, Jakshara S, Joshi C. Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress. Cell Stress and Chaperones, Available online 18 May 2018.
25. Abhilek Kumar Nautiyal, Arijit Jana, Sourya Bhattacharya, Tripti Sharma, Neha Bansal, Sree Sai Ogetiammini, Debashish Ghosh, Diptarka Dasgupta and Saugata Hazra: Microbial CYP450: An Insight into Its Molecular/Catalytic Mechanism, Production and Industrial Application. Principles and Applications of Fermentation Technology, 03rd Aug, 2018, 189-215
26. Neha Bansal, Mahesh B Khot, Arijit Jana, Abhilek K Nautiyal, Tripti Sharma, Diptarka Dasgupta, Swati Mohapatra, Sanoj Kumar Yadav, Debashish Ghosh and Saugata Hazra: Oleaginous Yeasts: Lignocellulosic Biomass Derived Single Cell Oil as Biofuel Feedstock. 03rd Aug, 2018, 263-306.
27. Pal M, Bhattacharya S, Kalyan G, Hazra Saugata. Cadherin profiling for therapeutic interventions in Epithelial Mesenchymal Transition (EMT) and tumorigenesis. Experimental Cell Research, Available online 16 April 2018.
28. Singh R, Sengar G, Singh U, Deb R, Junghare V, Hazra Saugata, Kumar S, Tyagi S, Das A, Raja T, Kumar A.Functional proteomic analysis of crossbred (Holstein Friesian × Sahiwal) bull spermatozoa. Reproduction in Domestic Animals. 2018.
29. Bandhu S, Khot M, Sharma T, Sharma O, Dasgupta D, Mohapatra S, Hazra Saugata, Khatri O, Ghosh D. Single Cell Oil from Oleaginous Yeast Grown on Sugarcane Bagasse-Derived Xylose: An Approach toward Novel Biolubricant for Low Friction and Wear. ACS Sustainable Chemistry & Engineering. 2017;6(1):275-283.
30. Bandekar D, Chouhan O, Mohapatra S, Hazra M, Hazra Saugata, Biswas S. Putative protein VC0395_0300 from Vibrio cholerae is a diguanylate cyclase with a role in biofilm formation. Microbiological Research. 2017; 202:61-70.
31. Das V, Kalyan G, Hazra Saugata, Pal M. Understanding the role of structural integrity and differential expression of integrin profiling to identify potential therapeutic targets in breast cancer. Journal of Cellular Physiology. 2017;233(1):168-185.
32. Bharatiy S, Hazra M, Paul M, Mohapatra S, Samantaray D, Dubey R, Sanyal S, Datta S, Hazra Saugata. In Silico Designing of an Industrially Sustainable Carbonic Anhydrase Using Molecular Dynamics Simulation. ACS Omega. 2016;1(6):1081-1103.
33. Wang W, McKinnie S, Farhan M, Paul M, McDonald T, McLean B, Llorens-Cortes C, Hazra Saugata, Murray A, Vederas J, Oudit G. Angiotensin-Converting Enzyme 2 Metabolizes and Partially Inactivates Pyr-Apelin-13 and Apelin-17Novelty and Significance. Hypertension. 2016;68(2):365-377.
34. Chouhan O, Bandekar D, Hazra M, Baghudana A, Hazra Saugata, Biswas S. Effect of site-directed mutagenesis at the GGEEF domain of the biofilm forming GGEEF protein from Vibrio cholerae. AMB Express. 2016;6(1).
35. Hazra Saugata, Kurz S, Wolff K, Nguyen L, Bonomo R, Blanchard J. Kinetic and Structural Characterization of the Interaction of 6-Methylidene Penem 2 with the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2015;54(36):5657-5664.
36. Kurz S*, Hazra Saugata*, Bethel C, Romagnoli C, Caselli E, Prati F, Blanchard J, Bonomo R. Inhibiting the β-Lactamase of Mycobacterium tuberculosis (Mtb) with Novel Boronic Acid Transition-State Inhibitors (BATSIs). ACS Infectious Diseases. 2015;1(6):234-242. (* contributed equally)
37. Basu R, Hazra Saugata, Shanks M, Paterson D, Oudit G. Novel Mutation in Exon 14 of the Sarcomere Gene MYH7 in Familial Left Ventricular Noncompaction With Bicuspid Aortic Valve. Circulation: Heart Failure. 2014;7(6):1059-1062.
38. Hazra Saugata, Xu H, Blanchard J. Tebipenem, a New Carbapenem Antibiotic, Is a Slow Substrate That Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2014;53(22):3671-3678.
39. Hadi T*, Hazra Saugata*, Tanner M, Blanchard J. Structure of MurNAc 6-Phosphate Hydrolase (MurQ) from Haemophilus influenzae with a Bound Inhibitor. Biochemistry. 2013;52(51):9358-9366. (* contributed equally)
40. Kurz S, Wolff K, Hazra Saugata, Bethel C, Hujer A, Smith K et al. Can Inhibitor-Resistant Substitutions in the Mycobacterium tuberculosis β-Lactamase BlaC Lead to Clavulanate Resistance?: a Biochemical Rationale for the Use of β-Lactam–β-Lactamase Inhibitor Combinations. AAC. 2013;57(12):6085-6096.
41. Paul M, Hazra M, Barman A, Hazra Saugata. Comparative molecular dynamics simulation studies for determining factors contributing to the thermostability of chemotaxis protein “CheY”. JBSD. 2013;32(6):928-949.
42. Quartararo C, Hazra Saugata, Hadi T, Blanchard J. Structural, Kinetic and Chemical Mechanism of Isocitrate Dehydrogenase-1 from Mycobacterium tuberculosis. Biochemistry. 2013;52(10):1765-1775.
43. Xu H, Hazra Saugata, Blanchard J. NXL104 Irreversibly Inhibits the β-Lactamase from Mycobacterium tuberculosis. Biochemistry. 2012;51(22):4551-4557.
44. Hazra Saugata, Szewczak A, Ort S, Konrad M, Lavie A. Post-Translational Phosphorylation of Serine 74 of Human Deoxycytidine Kinase Favors the Enzyme Adopting the Open Conformation Making It Competent for Nucleoside Binding and Release. Biochemistry. 2011;50(14):2870-2880.
45. Hazra Saugata, Konrad M, Lavie A. The Sugar Ring of the Nucleoside Is Required for Productive Substrate Positioning in the Active Site of Human Deoxycytidine Kinase (dCK): Implications for the Development of dCK-Activated Acyclic Guanine Analogues. JMC. 2010;53(15):5792-5800.
46. Hazra Saugata, Ort S, Konrad M, Lavie A. Structural and Kinetic Characterization of Human Deoxycytidine Kinase Variants Able To Phosphorylate 5-Substituted Deoxycytidine and Thymidine Analogues. Biochemistry. 2010;49(31):6784-6790.
47. Hazra Saugata, Sabini E, Ort S, Konrad M, Lavie A. Extending Thymidine Kinase Activity to the Catalytic Repertoire of Human Deoxycytidine Kinase. Biochemistry. 2009;48(6):1256-1263.
48. Sabini E, Hazra Saugata, Konrad M, Lavie A. Elucidation of Different Binding Modes of Purine Nucleosides to Human Deoxycytidine Kinase. JMC. 2008;51(14):4219-4225.
49. Sabini E, Hazra Saugata, Ort S, Konrad M, Lavie A. Structural Basis for Substrate Promiscuity of dCK. JMB,2008;378(3):607-621.
50. McSorley T, Ort S, Hazra Saugata, Lavie A, Konrad M. Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues. FEBS Letters. 2008;582(5):720-724.
51. Sabini E, Hazra Saugata, Konrad M, Lavie A. Nonenantioselectivity Property of Human Deoxycytidine Kinase Explained by Structures of the Enzyme in Complex with L- and D-Nucleosides. JMC. 2007;50(13):3004-3014.
52. Sabini E, Hazra Saugata, Konrad M, Burley S, Lavie A. Structural basis for activation of the therapeutic L-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase. NAR. 2006;35(1):186-192.
Books Authored
Book Chapters:
Patents
Detail of patents:
Patent1: Identification of functional internal ribosomal entry sites (IRES) at bovine heat shock protein 90 (Application Number: 201711042562)
Patent2: A METHOD OF PRODUCING THE POLYHYDROXYALKANOATE (PHA) USING WASTE WATER: (Application No. 202011031817)
Patent3: BL-Tester: An easy, cheap and reliable culture free point-of-care (ERC-PoC) diagnosis to detect antimicrobial resistance (Application No. 202111003064)
Databases & Web server
Hazra group is constantly working on development of programs which would facilitates biologist without computational expertise to use those tools to analyze and process big data. Following are few of our servers
Antihypertensive Peptide Database:
http://hazralab.iitr.ac.in/ahdb/index.php
AHPP:
http://hazralab.iitr.ac.in/ahtpg.php
AMPGP:
http://hazralab.iitr.ac.in/ampgp.php
mi-RNA Database:
http://hazralab.iitr.ac.in/dmircattle/index.php
MAT:
mat.iitr.ac.in
