Spotlight
Faculty
Departmental Facilities
Students
Research Scholars
Major Research Areas
Ongoing Projects
Pravindra Kumar
Pravindra Kumar Associate Professor kumarfbs[at]iitr.ac.in 91-1332-285072
Areas of Interest
  • Protein-Protein Interactions, Protein Engineering, Structure based drug design, Crystallography, Infectious Diseases, Drug Resistance, Waste degradation, Plant therapeutic proteins
Professional Background
FromToDesignationOrganisation
20012005Post-Doctoral Research AssociatePurdue University (U.S.A.)
19961999Senior DemonstratorAll India Institute of Medical Sciences
Honors and Awards
AwardInstituteYear
ASM- Professorship, 2017American Society of Microbiology and INDO-US forum2017
National Bioscience AwardDBT2015
Indo-US grant by IUSSTFIUSSTF2014
BOYSCAST FellowshipDepartment of Science and Technology, Govt. of India2008
Gold Medal for Best Ph.D. Award ( SV Talekar Award) All India Institute of Medical Sciences (A.I.I.M.S.)2001
Awarded the CSIR Senior Research fellowship All India Institute of Medical Sciences1999
Educational Details
DegreeSubjectUniversityYear
Ph.D.Biophysics (Structural Biology)All India Institute of Medical Sciences- New Delhi2001
M.Sc.Physics (Electronics)C.C.S. University, Meerut1995
Administrative Background
FromToDesignationOrganisationLevel
2017cont.Associate Dean (Corporate Interaction)I.I.T. Roorkee
20132016Chief Advisor SportsSports Council, I.I.T. ROORKEE
20102013Deputy Chief Advisor Students ClubI.I.T. Roorkee
20062008WardenI.I.T. Roorkee
Sponsored Research Projects
TopicFunding AgencyYear
Structural studies on 11S globulinDBT2015
Structural characterization of FabI ICMR2016
Structural characterization of MurA enzymeDRDO2016
Studies on Phthalate dioxygenaseNational bioscience2017
Structural studies of chorismate synthaseCSIR2013
Str. studies on MurA enzymeDRDO2017
Structure-based development of anti-bacterial enzyme inhibitors against enzyme involved in lipooligoICMR2012
Microbial degradation of toxic aromatic compounds using structural biology approachDRDO2012
Structural analysis of DAHP synthase from Arabidopsis thalianaDST2013
Structural Characterization Of DAHP Synthase For Designing Rational Inhibitors As Antibact. DrugMHRD2011
Structural studies of biphenyl dehydrogenase from Comamonas testosteroni strain B-356CSIR2011
Structural Studies of Aromatic-ring-Hydroxylating Dioxygenasees and their complexes with toxic PolyaDST2009
Memberships
  • American Society of Microbiology, MEMBER
  • AMERICAN CRYSTALLOGRAPHIC ASSOCIATION, MEMBER
  • INDIAN CRYSTALLOGRAPHIC ASSOCIATION, MEMBER
  • American Society of Microbiology, Member
Teaching Engagements
TitleCourse CodeClass NameSemester
Structural BiologyBT-204B.Tech. (Biotechnology) Spring
Structural BiologyBT-M.Sc.Spring
Macromolecular CrystallographyIBT02B.TechSpring
BioinformaticsBT 217B.TechAutumn
BioinformaticsBT 614M.Sc.Spring
Macromolecular CrystallographyIBT02B.TechAutumn
Research Scholar Groups
Scholar NameInterest
Monica SharmaComputational Biology
Mr. JaikrishnaStructural characterization of enzymes involved in bioremediation
Ms. Poonam DhanakarStructural biology of gram negative bacterial enzymes
Mr. Vikram DalalStructural studies of new drug targets from bacteria
Ms. NeetuStructural Biology of Wrightia Tinctoria proteins
Ms. Pooja KesariStructural and functional studies on plant proteins of medicinal importance
Ms. Aanchal SharmaStructural studies of drug targets from leishmania donavani
Ms. Neha SinghStructural studies of enzymes involved in pthalate degradation
Mr. Vijay SharmaStructural studies on shikimate and fatty acid biosynthetic pathway
Mr. Madhsudhanrao K.Structural studies of drug targets from Leishmania donavani
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Year
Structural studies on some mettaloproteins: Biphenyl dioxygenase and Dahp synthaseSushmita BhattacharyaA2006
Structural characterization of chitinase.Manali DattaA2006
Structural studies of trypsin inhibitor and its complex with trypsin and Factor XaDipak N. PatilA2007
Structure-function studies of Miraculin-like ProteinsP SelvakumarA2007
Structural studies on 11S globulin and 2S albumin from Wrightia tinctoriaPramod KumarA2009
Structural studies of drug targets fromdrug resistant pathogenic bacteriaShivendra PratapA2008
Structural studies on MurA and type II chorismate mutaseAditya DevA2008
Structural studies of enzymes involved in bioremediationSonali DhindwalA2009
Studies on peroxiredoxin anti-oxidant system from Candidatus liberibacter asiaticusMs. Anamika SinghA2009
Invitations
TopicOrganistaionCategoryYear
Purification and structural characterization of an 11S globulin from Wrightia tinctoria at Delhi-2015 2nd Plant Proteomics Workshop, Department of Botany, University of DelhilT2015
INDO-FRENCH seminar on the topic “Application of Structural Biology in Translational Research & Structure- Guided- Drug- Design at ACTREC-Navi Mumbai, November 19-20, 2015 on "Active-Site Plasticity is Essential to Carbapenem Hydrolysis by OXA-58 Class D ?-Lactamase of Acinetobacter Baumannii"ACTREC Navi MumbaiT2015
Indo-US International Conference/workshop on "Recent Advances in Structural Biology and Drug Discovery,9-11 October 2014, IIT Roorkee“Ligand bound structures of 3-deoxy- D-manno-octulosonate 8-phosphate phosphatase fromMoraxella catarrhalis reveals water channel connecting the active site for second step of catalysis” at IIT RoorkeeIndo-US at IIT RoorkeeT2014
Structural studies of enzymes involved in biphenyl degrading pathway, at JNU-201342nd National Seminar on Crystallography, JNU DelhiT2013
National Conference on Recent Trends in Protein Structural Biology will be held during December 16-18, 2013 at Jamia Millia Islamia on " structural studies of enzymes involved in the biodegradation oftoxic aromatic compounds"Jamia Millia Islamia, DelhiT2013
Structural Proteomics of therapeutic proteins in plants at Delhi 20131st Plant Proteomics workshop, Department of Botany, University of DelhiT2013
Invited for a lecture for "Current Trends in Structural Biology (CTSB)- 2012", held during 2nd April 2012, Department of Biophysics, AIIMS;"Biochemical studies and ligand-bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme"All India Institute of Medical Sciences DelhiI2012
in Proteomics-2012 held in Las Vegas, USA during July 02-04, 2012 on "Structural analysis of chorismate synthase from Plasmodium falciparum: a novel target for antimalaria drug discovery"Proteomics-2012 held in Las Vegas, USA T2012
Indo-US symposium on "Modern Trends in Macromolecular Structires" Feb 21-24th 2011 held IIT BombayIIT BombayI2011
"Lactoferrin or Transferrin? Crystal Structure of Lactoferrin" Purdue University (U.S.A.)T2002
"Structure solution of a 40 kDa protein from goat secretions". at discussion meeting on Macromolecular crystallography Feb 6-7, 1998. at MBU, IISc. Banglore.IISC BangaloreI1998
Participation in short term courses
Couse NameSponsored ByDate
‘Hands-on-Training on Modern Techniques in Biotechnology (Nano/structural biotechnology)’USBDJan 2013
Workshop for National Science Talent Scholarship in RoorkeeNCERT2012
Courses or Conferences Organised
Conference NameSponsored ByDate
Indo-US conference on "Structural Biology and Drug Discovery"IUSSTF, DRDO, DST, ICMR, INSA9-12, 2014
Macromolecular CrystallographyI.I.T. Roorkee31-10-2010
National International Collaboration
TopicOrganisationLevel
Drug resistanceDr. Dasantila Kotra, York University, Toronto, CanadaRP
Protein engineeringProf. Michel Sylvestre:Centre INRS–Institut Armand-FrappierRP
Plant hemoglobinProf. Suman Kundu:Delhi University (South Campus)RP
Globulins from Wrightia tinctoria and other plant proteinsDr. Girish Mishra:Delhi University (North Campus)RP
Protein from momordica charantiaNBRI LucknowRP
Biodegradation of toxic aromatic compoundsProf. J.T. Bolin: Purdue University (U.S.A.)RP
Books Authored
Biodegradation And Bioremediation of the series  Env. Sci. & Engg.

Environmental Pollution:  Monitoring, Modeling and Control

 

Refereed Journal Papers

  1. Pratap S,Dev A, Sharma V, Yadav R, Narwal M, Tomar S, Kumar P,(2017) Structure of Chorismate Mutase-like Domain of DAHPS from Bacillus subtilis Complexed with Novel Inhibitor Reveals Conformational Plasticity of Active Site    Scientific Reports, 7(1):6364. DOI: 10.1038/s41598-017-06578-1.

  2. Kumar P, Kesari P, Dhindwal S, Choudhary A.K., Katiki M, Neetu, Verma A, Ambatipudi K, Tomar S, Sharma A.K., Mishra G, Kumar P (2017). A novel function for globulin in sequestering plant hormone:Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin. Scientific Reports 7(1):4705.DOI:10.1038/s41598-017-04518-7.

  3. Singh N, Dalal V, Mahto JK, Kumar P. (2017) Biodegradation of phthalic acid esters (PAEs) and in silico structural characterization of mono-2-ethylhexyl phthalate (MEHP) hydrolase on the basis of close structural homolog.J Hazard Mater. 2017 Apr 25;338:11-22. doi: 10.1016/j.jhazmat.2017.04.055.PMID:28531656

  4. Sharma A, Kumar P, Kesari P, Chaudhary N, Katiki M, Mishra M, Singh PK, Gurjar, BR, Sharma AK, Tomar S, Kumar P. (2017) Purification and Characterization of 2S albumin from seeds of Wrightia tinctoria exhibiting Antibacterial and DNase Activity. Protein Pept Lett. Jan 26. [Epub ahead of print] PubMed PMID: 28128054.

  5. Pratap S, Kesari, P, Yadav R, Dev A, Narwal M & Kumar P (2017). Acyl chain preference and inhibitor identification of Moraxella catarrhalis LpxA: Insight through crystal structure and computational studies. Int J Biol Macromol. (in Press)

  6. Kesari P, Chaudhary N, Sharma A, Katiki M, Kumar P, Gurjar BR, Tomar S, Sharma AK, Kumar P (2016). Structural, Functional and Evolutionary Aspects of Seed Globulins. Protein Pept Lett. 2016 Dec 20. [Epub ahead of print] PubMed PMID: 28000570.

  7. Dhindwal S, Kesari P, Singh H, Kumar P, Tomar S (2016). Conformer and pharmacophore based identification of peptidomimetic inhibitors of chikungunya virus nsP2 protease. J Biomol Struct Dyn. 2016 Dec 2:1-18. [Epub ahead of print] PubMed PMID: 27844505.

  8. Tomar JS, Narwal M, Kumar P, Peddinti RK (2016). Characterization of substrate binding and enzymatic removal of a 3-methyladenine lesion from genomic DNA with TAG of MDR A. baumannii. Mol Biosyst. 2016 Oct 18;12(11):3259-3265. PubMed PMID: 27714027.

  9. Sharma R, Fatma B, Saha A, Bajpai S, Sistla S, Dash PK, Parida M, Kumar P,Tomar S. (2016)  Inhibition of chikungunya virus by picolinate that targets viral capsid  protein. Virology. 2016 Sep 8;498:265-276. doi: 6.1016/j.virol.2016.08.029.[Epub ahead of print] PubMed PMID: 27614702.

  10. Batra M, Sharma R, Malik A, Dhindwal S, Kumar P, Tomar S. (2016) Crystal structure of pentapeptide-independent chemotaxis receptor methyltransferase (CheR) reveals idiosyncratic structural determinants for receptor recognition. J Struct Biol.2016 Aug 17. pii: S1047-8477(16)30174-5. doi: 10.1016/j.jsb.2016.08.005. [Epub ahead of print] PubMed PMID: 27544050.

  11. Dhindwal S, Gomez-Gil L, Neau DB, Pham TT, Sylvestre M, Eltis LD, Bolin JT, Kumar P. (2016) The structural basis of the enhanced pollutant-degrading capabilities of an engineered biphenyl dioxygenase. J Bacteriol.  198(10):1499-512. 

  12. Mukhi N, Dhindwal S, Uppal S, Kapoor A, Arya R, Kumar P, Kaur J, Kundu S. (2016) Structural and Functional Significance of the N- and C-Terminal Appendages in Arabidopsis Truncated Hemoglobin. Biochemistry. 55(12):1724-40.

  13. Pratap S, Katiki M, Gill P, Kumar P*, Golemi-Kotra D. (2015) Active-Site Plasticity is Essential to Carbapenem Hydrolysis by OXA-58 Class D β-Lactamase of Acinetobacter baumannii. Antimicrob Agents Chemother. 60(1):75-86. (* Corresponding  author)

  14. Kesari P, Patil DN, Kumar P, Tomar S, Sharma AK, Kumar P. (2015) Structural and functional evolution of chitinase- like-proteins from plants. Proteomics. 15(10):1693-705. 

  15. Dhindwal S, Priyadarshini P, Patil DN, Tapas S, Kumar P, Tomar S, Kumar P.(2015) Ligand-bound structures of 3-deoxy-D-manno octulosonate 8-phosphate phosphatase from Moraxella catarrhalis reveal a water channel connecting the active site for the second step of catalysis. Acta Crystallogr D Biol Crystallogr. 71(Pt 2):239-55.

  16. Sharma N, Selvakumar P, Bhose S, Ghosh DK, Kumar P, Sharma AK. (2015) Crystal structure of a periplasmic solute binding protein in metal-free, intermediate and metal-bound states from Candidatus Liberibacter asiaticus. J Struct Biol.189(3):184-94. 

  17. Aggarwal M, Dhindwal S, Kumar P, Kuhn RJ, Tomar S. (2014) trans-Protease activity and structural insights into the active form of the alphavirus capsid protease. J Virol. 2014 Nov;88(21):12242-53.

  18. Selvakumar P, Sharma N, Tomar PP, Kumar P, Sharma AK. (2013) Structural insights into the aggregation behavior of  Murraya koenigii miraculin-like protein below pH 7.5. Proteins. 82(5):830-40..

  19. Dev,  A., Bodra, N. Kumar, P., Pratap, S, Kumar, P. (2013) Homology modeling and functional characterization of three-dimensional structure of DAHP Synthase from Brachypodium distachyon. J. Proteins Proteom. 4(1). (In Press).

  20. Patil DN, Datta M, Dev A, Dhindwal S, Singh N, Dasauni P, Kundu S, Sharma AK, Tomar S, Kumar P. (2013) Structural Investigation of a Novel N-Acetyl Glucosamine Binding Chi-Lectin Which Reveals Evolutionary Relationship with Class III Chitinases. PLoS One. 23;8(5):e63779. [Epub ahead of print]

  21. Mukhi N, Dhindwal S, Uppal S, Kumar P, Kaur J, Kundu S. (2013) X-Ray crystallographic structural characteristics of              Arabidopsis hemoglobin I and their functional implications. Biochim Biophys Acta. [Epub ahead of print]

  22. Preeti, Tapas S, Kumar P, Madhubala R, Tomar S. (2013) Structural insight into DFMO resistant ornithine decarboxylase from Entamoeba histolytica: an inkling to adaptive evolution. PLoS One. (In Press)

  23. Colbert CL1, Agar NY1Kumar P1, Chakko MN, Sinha SC, Powlowski JB, Eltis LD, Bolin JT. (2013) Structural characterization of Pandoraea pnomenusa B-356 biphenyl dioxygenase reveals features of potent polychlorinated biphenyl-degrading enzymes. PLoS One. 8(1):e52550. (1Contributed equally)

  24. Aggarwal M, Tapas S, Preeti, Siwach A, Kumar P, Kuhn RJ, Tomar S. (2013) Crystal structure of aura virus capsid protease and its complex with dioxane: new insights into capsid-glycoprotein molecular contacts. PLoS One.7(12):e51288.

  25. Patil, D.N., Chaudhry, A., Sharma, A.K., Tomar, S., Kumar, P. (2012). Structural basis for dual inhibitory role of tamarind Kunitz inhibitor (TKI) against factor Xa and trypsin. FEBS J  279(24):4547-64. 

  26. Kumar, P., Patil, D.N., Chaudhary, A., Tomar, S.,Yernool, D., Singh, N., Dasauni, P.,  Kundu, S., & Kumar, P., (2012) Purification and biophysical characterization of 11S globulin from Wrightia tinctoria exhibiting hemagglutinating activity. Protein & peptide letters 20(5):499-509.

  27. Kumar P, Mohammadi M, Dhindwal S., Bolin JT & Sylvestre M. (2012) Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase BBRC. 421(4):757-62.

  28. Pratap S, Narwal M, Dev A, Dhindwal S, Tomar S, Kumar P (2012) Crimean- Congo hemorrhagic fever virus:  Strategies to combat with an emerging threat to human. Current Bioinformatics. 7 (4), 466-477.

  29. Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, LD., Bolin J.T. & Sylvestre M. (2011). Structural Insight into the Expanded PCB-Degrading Abilities of a Biphenyl Dioxygenase Obtained by Directed Evolution. J Mol Biol. 405(2):531-47.

  30. Dev, A., Tapas,S.,  Pratap,S.,  Kumar, P. (2012) Structure and Function of Enzymes of Shikimate Pathway. Current Bioinformatics 7 (4), 374-391.

  31. Preeti, Tapas S, Kumar P, Madhubala R, Tomar S. (2012) Biochemical, Mutational and In Silico Structural Evidence for  Functional Dimeric Form of the Ornithine Decarboxylase from Entamoeba histolytica. PLoS Negl Trop Dis. 6(2):e1559. 

  32. Narayanan A, Paul LN, Tomar S, Patil DN, Kumar P, Yernool DA. (2012) Structure-function studies of DNA binding domain of response regulator KdpE reveals equal affinity interactions at DNA half-sites. PLoS One.7(1):e30102. 

  33. Aggarwal, M., Dhindwal, S., Pratap, S.,  Kuhn, R.J.,  Kumar P., & Tomar, S. (2011). Crystallization, high- resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane. Acta Cryst. F67, 1394-1398.

  34. Bhattacharya, S., & Kumar, P. (2011). An insilico approach to structural elucidation of 3-deoxy-d arabino-heptulosonate 7-phosphate synthase from Arabidopsis thaliana: Hints for herbicide design. Phytochemistry. 73(1):7-14.

  35. Dhindwal, S., Patil, D.N., Mohammadi, M., Sylvestre, M., Tomar, S., Kumar, P. (2011). Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme. J Biol Chem. 286(42):37011-22.

  36. Tapas, S., Kumar, A., Dhindwal, S., Preeti, Kumar, P. (2011). Structural analysis of chorismate synthase from Plasmodium falciparum: A novel target for antimalaria drug discovery. Int J Biol Macromol. 49(4), 767-77.

  37. Mohammadi, M.†, Viger, J.F.,†, Kumar, P.,†, Barriault, D., Bolin, J.T., & Sylvestre, M. (2011).  Fine tuning  Rieske -type oxygenases reactive atoms to expand their substrate range. J. Biol. Chem. 286(31):27612-35. (†Contributed equally).

  38. Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, LD., Bolin J.T. & Sylvestre M. (2011).Structural Insight into the Expanded PCB-Degrading Abilities of a Biphenyl Dioxygenase Obtained by Directed Evolution. J Mol Biol. 405(2):531-47.

  39. Kumar, P., Gómez-Gil, L., Mohammadi, M., Sylvestre, M., Eltis, L.D. & Bolin, J.T. (2011). Anaerobic crystallization and initial X-ray  diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals. Acta Crystallogr Sect F, 67, 59-62.

  40. Sakshi, Patil, D.N., Tomar, S., Sylvestre, M., & Kumar, P. (2010). Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356. Acta Crystallogr Sect F , 66:1517-20.

  41. Gahloth, D., Selvakumar, P., Shee, C., Kumar, P*., Sharma., A.K. (2010). Cloning, sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii. Arch Biochem Biophys. 1;494(1):15-22. (Corresponding author)

  42. Tomar, S., Patil, D.N., Datta, M., Tapas, S., Preeti, Chaudhary, A., Sharma, A.K., Tomar, S., Kumar, P. (2009). Crystallization and preliminary X-ray diffraction analysis of the complex of Kunitz-type tamarind trypsin inhibitor and porcine pancreatic trypsin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 1;65(Pt 11):1179-81.

  43. Patil, D.N., Datta, M., Chaudhary, A., Tomar, S., Sharma, A.K. & Kumar, P. (2009). Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 4):343-5. 

  44. Patil, D.N., Preeti, Chaudhry, A., Sharma, A.K., Tomar, S., Kumar, P. (2009). Purification, crystallization and preliminary crystallographic studies of a Kunitz-type proteinase inhibitor from tamarind (Tamarindus indica) seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 7):736-8.

  45. Nath, M., Kumar, P., & Sulaxna. (2009). Structural Investigation of (5-Amino-1,3,4-thiadiazolyl-2-thionato)trimethyltin(IV): 1D Chains Generated by Hydrogen Bonding. SPECTROSCOPY LETTERS, 42, 268-273.

  46. Nath, M., Singh, H., Kumar, P., Kumar, A., Song, X. and Eng, G. (2009), Organotin(IV) tryptophanylglycinates:potential non-steroidal antiinflammatory agents; crystal structure of dibutyltin(IV) tryptophanylglycinate. Applied Organometallic Chemistry, 23: 347–358.

  47. Chaudhary, N.S., Shee, C., Islam, A., Ahmad, F., Yernool, D., Kumar, P. & Sharma, A.K. (2008). Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii seeds. Phytochemistry. 69(11):2120-6.

  48. Shee, C., Singh, T.P., Kumar, P. & Sharma, A.K.  (2007). Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor. Acta Crystallogr Sect F Struct Biol Cryst Commun..63(Pt 4):318-9.

  49. Horsman, G.P., Bhowmik, S., Seah, S.Y., Kumar, P., Bolin, J.T. & Eltis, L.D. (2007). The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad. J Biol Chem. 282(27):19894-904.

  50. Gomez-Gil L., Kumar, P., Barriault, D., Bolin, J.T., Sylvestre, M. & Eltis, L.D. (2007). Characterization of biphenyl dioxygenase of Pandoraea pnomenusa B-356 as a potentpolychlorinated biphenyl-degrading enzyme. J Bacteriol.189(15):5705-15.

  51. Dey, S., Vijayaraghavan, R., Goel, V.K., Kumar, S.,  Kumar, P. & Singh, T.P. (2004) Design of a model peptide with a, b-dehydro-residues: Synthesis of Boc-Ile- DAla-OCH3 and its crystal structures obtained from two different solvents. J. Mol.  Struct. 737 27-3.

  52. Vijayaraghavan, R., Makker, J., Kumar, P., Dey, S., Singh, T. P. (2003) Crystal structure of Boc-Trp (CHO) - ΔPhe-Ile-ΔPhe-Leu-OCH3, C48H59N6O10. Zeitschrift fuer Kristallographie - New Crystal Structures,    218(1), 52-54. 

  53. Makker, J., Dey, S., Kumar, P., Singh, T. P. (2003) Crystal structure of Boc-ILe-ΔPhe-Ile-OCH3, C27H43N3O7. Zeitschrift fuer Kristallographie - New Crystal Structures (2003),  218, 179-180. 

  54. Vijayaraghavan R., Makker, J., Kumar, P., Dey S., Singh T.P. (2003). Design of peptides with a, b -dehydro-residues: syntheses, crystal structures and molecular conformations of two ΔPhe-Trp containing   peptides: Journal of Molecular Structure,  654(1-3),  103-110.

  55. Makker, J., Dey, S., Mukherjee, S., Vijayaraghavan, R., Kumar, P., Singh, T. P. (2003). Design of peptides with  a, b  -dehydro-residues: synthesis, crystal structure and molecular conformation of a tetrapeptide Z-ΔVal-Val-ΔPhe-Ile-Ome. Journal of Molecular Structure, 654(1-3), 119-124.

  56. Vijayaraghavan R., Kumar P., Dey S., Singh T.P. (2003). Design of peptides with branched beta-carbon dehydro- residues: syntheses, crystal structures and molecular conformations of two peptides, (I) N-Carbobenzoxy-DeltaVal-Ala-Leu-OCH3 and (II) N-Carbobenzoxy-DeltaIle-Ala-Leu-OCH3 : J. Pept. Res. 62(2):63-9.

  57. Mohanty A.K., Singh G., Paramasivam M., Saravanan K., Jabeen T., Sharma S., Yadav S., Kaur P., Kumar P., Srinivasan A, Singh  T.P (2003). Crystal structure of a novel regulatory 40-kDa mammary gland protein (MGP-40) secreted during involution : J. Biol. Chem. 278(16):14451-60.

  58. Makker, J., Dey, S., Mukherjee, S., Kumar, P., Singh, T. P. (2002). Crystal structure of Boc-Leu-ΔPhe-ΔPhe-Ile-OCH3,  C36H48N4O7. Zeitschrift fuer Kristallographie-New Crystal Structures (2002), 217(3), 372-374.   

  59. Makkar, J., Dey, S., Kumar, P., Singh, T.P. (2002). Crystal structure of Boc-Leu-ΔPhe-Ile-ΔPhe-Ile-OCH3,C42H59N5O8. Zeitschrift fuer Kristallographie - New Crystal Structures, 217(3), 369-371.

  60. Kumar, P., Yadav, S. & Singh, T.P. (2002). Crystallization and structure determination of goat lactoferrin at 4.0 Å  resolution: A new form of packing in lactoferrins with a high solvent in crystals. Ind. J. Biochem. Biophys. 39(1):16-21.

  61. Makkar, J., Dey, S., Kumar, P., Singh, T.P. (2002). Design of peptides with alpha, beta-dehydro residues: pseudo-tripeptideN-benzyloxycarbonyl-DeltaLeu-L-Ala-L-Leu-OCH3. Acta Crystallogr C58, 212-4. 

  62. Kumar, P., Khan, J.A., Yadav, S. & Singh, T.P. (2002). Crystal structure of equine apolactoferrin at 303 K providing further evidence of closed conformations of N and C lobes.  Acta Crystallogr  D58, 225-32.

  63. Singh, J.D., Milton, M.D., Bhalla, G. Khandelwal, B. L., Kumar, P., Singh, T. P., Butcher, R. J. (2001). Design, synthesis and structural aspects of acyclic N3E2 (E = Se or Te) type donors and its complexes with Group 12 metals.Phosphorus, Sulfur and Silicon and the Related  Elements. (171-172)  477-484.

  64. Milton, M.D., Singh, J. D., Khandelwal, B. L.; Kumar, P., Singh, T. P., Butcher, R. J. (2001). Design, synthesis and  structural aspects of terdentate (N,O,Se/Te) donors and their competitive coordination behavior towards Pt(II). Phosphorus,Sulfur and Silicon and the Related Elements, (171-172)  485-492. 

  65. Tomar, S., Yadav, S., Chandra, V., Kumar, P., Singh, T.P. (2001). Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus) : Acta Crystallogr  D57, 1669-70.

  66. Vijayaraghavan, R., Kumar, P., Dey, S., Singh, T.P. (2001). Design of peptides with alpha, beta-dehydro residues: a dipeptide with a branched beta-carbon dehydro residue at the (i+1) position, methyl N - (benzyloxycarbonyl) - alpha, beta - didehydrovalyl - L - tryptophanate: Acta Crystallogr C57, 1220-1.

  67. Khan, J.A., Kumar, P., Sharma, S., Mohanty, A.K., Jabeen, T., Paramasivam, M., Yadav, S., Srinivasan, A. & Singh, T.P. (2001). Mechanism of iron-uptake and iron-release in lactoferrins: Proc. Ind. Nat. Sc. Acad. B67, 1-17.

  68. Kumar, P., Yadav, S., Srinivasan, A., Bhatia, K.L. & Singh, T.P. (2001). A novel 40 kDa protein from goat mammary secretions: purification,  crystallization and preliminary X-ray diffraction studies: Acta Crystallagr. D57, 1332-3.

  69. Sharma, S., Kumar, P., Betzel, C., Singh, T.P. (2001). Structure and function of proteins involved in milk allergies: J Chromatogr BBiomed Sci Appl. 756(1-2), 183-7.

  70. Khan, J.A., Kumar, P., Paramsivam, M., Yadav, R.S., Sahni, M.S., Sharma, S., Srinivasan, A. & Singh, T.P. (2001). Structure of camel apolactoferrin at 2.6Å resolution and structural basis of its dual role as a transferrin-cum-lactoferrin: J. Mol. Biol.  309(3), 751-61. 

  71. Khan J.A., Kumar, P., Srinivasan, A., Singh, T.P. (2001). Protein intermediate trapped by the simultaneous crystallization process. Crystal structure of an iron-saturated intermediate in the Fe3+ binding pathway of camel lactoferrin at 2.7 Å  resolution: J.Biol. Chem.  276(39), 36817-23.

  72. Betzel, C., Gourinath, S., Kumar, P., Kaur, P., Perbandt, M., Eschenburg, S. & Singh, T.P. (2001). Structure of a serine protease proteinase K from Tritirachium album   Limber at 0.98Å resolution: Biochemistry. 40(10), 3080-8. 

  73. Bhatia, S., Kumar, P., Kaur, P., & Singh, T.P. (1999). Design of peptides with a ,b - dehydro-residues: synthesis, and crystal and molecular structure of a 310-helical tetrapeptide Boc-L-Val-D Phe-D Phe-L-Ile-OCH3: J. Pept. Res. 54, 249-256.

  74. Vijayaraghavan, R., Kumar, P., Dey, S. & Singh, T.P. (1998). Design of Peptides with a, b -Dehydro-residues: Synthesis, Crystal and Molecular Conformation of N-Boc-L-Ile-D Phe-L-Trp-OCH3: J.Pept Res. 52, 89-96. 

 

Miscellaneous
  • 1. Macromolecular Crystallography: Institute elective 2. Bioinformatics: M.Sc. & B.Tech. (Biotechnology) 3. Structural Biology: B.Tech. (Biotechnology) 4. Structure based drug design: B.Tech. (Biotechnology) 5. protein crystallography: B.Tech. (Biotechnology)