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Debabrata Sircar
Debabrata Sircar Assistant Professor debsrfbt[at]iitr.ac.in (91)-1332 285540
Areas of Interest
  • ROS-omics, The use of genetics and genomics resources for the improvement of fruit quality of Rosaceous plant
  • Metabolic Pathway Engineering, Elucidation & engineering of secondary metabolic pathways in selected medicinal plants
  • Fruit Sensors, Prediction of fruit quality using non-invasive metabolite-based electronic sensors
  • Plant Metabolomics, Metabolomics of economically important plants for value addition
Professional Background
FromToDesignationOrganisation
20112013Post-doctoral FellowIPB, TU-Braunschweig, GERMANY
20102011Post-doctoral FellowUniversity of Evora, PORTUGAL
Honors and Awards
AwardInstituteYear
DAAD Research Stays for University Academics and Scientists IIT Roorkee2015
SERB Young ScientistSERB India2013
Post-doctoral fellowshipInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2011-13
FCT Post-doctoral felloship (with evaluation score 5/5))Laboratory of molecular biology, University of Evora; PORTUGAL2010
DAAD FellowshipInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2008
University First, gold medal in M.Sc BotanyThe University of Burdwan, West Bengal2004
University first in B.Sc BotanyVinoba Bhave University2001
Educational Details
DegreeSubjectUniversityYear
M.ScPlant ScienceUniversity of Burdwan, West Bengal2004
Ph.D.Plant BiologyIndian Institute of Technology Kharagpur2009
FCT, Post-doctoral fellowMolecular Plant Breeding researchUniversity of Evora, Portugal2010-2011
Post-doctoral fellowFunctional Genomics and Metabolomics researchInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2011-2013
Administrative Background
FromToDesignationOrganisationLevel
2015Till dateMember Secretary IBSCInstitutional Biosafety Committee
2015Till DateOfficer In-Charge AdministrationBiotechnology Department IIT Roorkee
2014Till DateOfficer In-Charge Store & PurchaseBiotechnology Department IIT Roorkee
2014Till DateMember DRC (Departmental Research Committee)Biotechnology Department IIT Roorkee
Sponsored Research Projects
TopicFunding AgencyYear
Functional Genomics of Phytoalexin Biosynthesis in AppleSERB Fast Track2014
Development of an Electronic Nose For the Optimum Harvesting time and Fruit Quality in AppleICAR NASF1918
Detection of Spoiled Coconut at Pre–Processing Stage by Portable Wireless Electronic Sensor.Coconut Development Board1918
Development of a rapid and robust high throughput reporter cell based bioassay for detection of xenoICAR NASF (as Co-PI)1918
Participation in seminars
NamePlaceSponsored ByDate
Recent Advances in Ayurvedic herbal medicine- from source to manufacturing (Invited Talk)Uttarakhand Ayurved University, Dehradun, Uttarakhand (India)IIT Roorkee16.09.2017
4th International Conf. and Exhibition on Pharmacognosy, Phytochemistry and Nat Prod (Invited Talk) Sao Paulo, BrazilIIT Roorkee29.08.2016
XV Annual convention of ISVPT (Invited Talk)NDRI, Karnal, Haryana (India) SERB Young Scientist Grant16.01.2016
International Conference on Molecular Signaling- Recent Trends in Biosciences (Invited Talk)North Eastern Hill University, Shilong (India)IIT Roorkee22.11.2015
Teaching Engagements
TitleCourse CodeClass NameSemester
Cell BiologyBTN 104B.TechSpring
Plant System PhysiologyBTN 642M.ScSpring
Plant BiotechnologyBTN 303B.TechAutumn
Cell and Tissue Cult TechnologyBTN 621M.ScAutumn
Recombinant DNA TechnologyBTN 657M.TechAutumn
Projects and Thesis Supervised
Title of ProjectNames of Students
PURIFICATION, STRUCTURE ELUCIDATION AND ASSESSMENT OF ANTI-PROLIFERATIVE PROPERTY OF A NOVEL BIPHENYMr. Aman Kr. Chaudhary (M.Sc)
DEVELOPING ALTERNATIVE OXIDASE (AOX) AS A FUNCTIONAL MARKER FOR ADAPTIVE CELL-REPROGRAMMING IN APPLEMs. Kriti Juneja
EVALUATION OF THE TOTAL PHENOLIC AND FLAVONOID CONTENT AS WELL AS THE ANTIOXIDANT PROPERTY OF CALLUSMs. Pallavi Roy Chowdhury
METABOLIC STUDIES OF INDIAN MEDICINAL PLANT RUTA GRAVEOLENS FOR RAPID ISOLATION OF COMMERCIALLY IMPOMr. Sagar Tyagi
PHYTOALEXINS BIOSYNTHESIS & BIOCHEMICAL CHARACTERIZATION OF BENZALDEHYDE DEHYDROGENASE IN PyruMs Annu Verma
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Year
BIOSYNTHESIS OF BENZOATE-DERIVED BIPHENYL PHYTOALEXINS IN CELL CULTURES OF Pyrus pyrifoliaDr. Shashank Sagar SainiA2013
BIOCHEMICAL AND MOLECULAR ANALYSES OF PHYTOALEXIN BIOSYNTHESIS IN APPLE CELL CULTURESDr. Sarkate Amol VitthalraoA2013
MOLECULAR ANALYSES OF CINNAMTE CoA-LIGASE FROM PHYTOALEXIN PRODUCING APPLE CELL CULTURESDr. Deepa TeotiaA2013
ISOLATION AND CHARACTERIZATION OF BIOACTIVE METABOLITES FROM SELECTED MEDICINAL PLANTSMs. Kriti JunejaO2015
METABOLOMICS-BASED APPROACHED TO UNRAVEL HEALTH PROTECTIVE PROPERTIES OF COCONUT PRODUCTSMr. Mukund KumarO2016
METABOLOMICS-BASED BIOACTIVITY ASSESSMENT OF PLUMBAGO ZEYLANICAMr. Ashwani KumarO2016
METABOLOMICS OF ASWAGANDHA FOR CHARACTERIZATION OF VALUE ADDED NATURAL PRODUCTS.Ms. VarshaO2016
VOLATILE METABOLOMICS OF RIPENING ASSOCIATED METABOLITES IN APPLE.Ms KomolO2017
VOLATOMICS OF PAPAYA TO DETERMINE ACCURATE HARVESTING TIMEMr. Waghmode BO2017
FUNCTIONAL GENOMICS AND METABOLOMICS OF HEAT STRESS IN BARLEYMs. ReekuO2014
DEVELOPEMNT OF ELECTRONIC NOSE SENSOR FOR PRE-PROCESSING DETECTION OF SPOILED COCONUTMr. Arun KumarO2017
Invitations
TopicOrganistaionCategoryYear
Linking R & D with stakeholders & EntrepreneursFRI, DehradunT2018
Precision Agriculture in India- Way ForwardICART2018
Research Stays for University Academics and Scientists 2015TU-Braunschweig, GERMANYI2015
Courses or Conferences Organised
Conference NameSponsored ByDate
International Conference on ‘Molecular Signaling: Recent Trends in Biomedical and Translational ResDBT, SERB, DRDO, INSA, NDRC17.12.2014
Indo-US conference and workshop on “Recent Advances on Structural Biology and Drug DiscoveryDBT, SERB, DRDO, INSA, NDRC and Indo-US09-10.2014
National International Collaboration
TopicOrganisationLevel
Metabolomics and Functional Genomics of Rosaceous Plants.TU-BS-GermanyRP
Sub-cellular Localization of phytolaexin biosynthesis proteins in RosaceaeInstitute of Plant Biology, TU-BS, GermanyRP
Alternative oxidase gene as functional marker candidate metabolic re-programming.University of Evora, PORTUGALRP
Health protective Phytomedicine from selected Indian medicinal plantsIIT KharagpurRP
Molecular breeding in apple and pearICAR-CITH, SrinagarRP
Metabolic flux analyses in natural product BiosynthesisIIT MandiRP
Books Authored

1 Juneja K, Saini SS, Gaid M, Beerhues L, Sircar D* (2017) Production of life-saving drugs from Himalayan herbs. Sustainable Utilization of Natural Resources (Mondal, P., Dalai, A.K., eds.), CRC Press, Boca Raton, USA Chapter 11:333-352.

2. Mitra A, Mukherjee C, Sircar D (2017) Metabolic Phytochemistry-Based Approaches for Studying Secondary Metabolism Using Transformed Root Culture Systems. In: Jha S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. Chapter 20: 513-37

3. Nogales A, Noceda C, Ragonezi C, Cardoso H G, Campos M D, Frederico A M, Sircar D, Kumar S R,  Polidoros A, Peixe A  and Arnholdt-Schmitt B  (2015) Functional marker development from  AOX  genes requires deep phenotyping and individualized diagnosis, in Alternative respiratory pathways in higher plants (eds K. J. Gupta, L. A.J. Mur and B. Neelwarne), John Wiley & Sons, Ltd, Chichester, UK. Chapter 13.1: 273-2080.

Refereed Journal Papers

19. Varshney R, Varshney R, Mishra R, Gupta S, Sircar D, Roy P (2018) Kaempferol alleviates palmitic acid-induced lipid stores, endoplasmic reticulum stress and pancreatic β-cell dysfunction through AMPK/mTOR-mediated lipophagy. Journal of Nutritional Biochemistry 57: 212-27

18. Sarkate A, Saini SS, Kumar P, Sharma AK, Sircar D* (2018) Salicylaldehyde synthase activity from Venturia inaequalis elicitor-treated cell culture of apple. J Plant Physiol 221: 66-73.

17. Liu B, Gaid M, Chizzali C, Khalil MNA, Sircar D, Reckwell, D, Beerhues  L (2018) Biphenyls and dibenzofurans are the phytoalexins of apple. (In Press)

16. Saini SS, Teotia D, Gaid M, Thakur A, Beerhues L, Sircar D* (2017) Benzaldehyde dehydrogenase-driven phytoalexin biosynthesis in elicitor treated Pyrus pyrifolia cell cultures. J Plant Physiol 215: 154-62.

15. Sarkate A, Banerjee S, Mir JI, Roy P, Sircar D* (2017) Antioxidant and cytotoxic activity of bioactive phenolic metabolites isolated from the yeast-extract treated cell culture of apple. Plant Cell Tissue Organ Culture

130:641-49.

14.Teotoa D, Saini SS, Gaid M, Beuerle T, Beerhues L, Sircar D * (2016) Development and Validation of a New HPLC Method for the Determination of Biphenyl and Dibenzofuran Phytoalexins in Rosaceae. J Chromatogr Sci (2016) doi: 10.1093/chromsci /bmw019.

13. Mukherjee C, Sircar D, Chatterjee M, Das S, Mitra A (2014) Combating photooxidative stress in green hairy roots of Daucus carota cultivated under light irradiation. J Plant Physiol 171: 179-87.

12. Sircar D, Gaid M, Chizzali C, Reckwell D, Kaufholdt D, Beuerle T, Broggini GA, Flachowsky H, Liu B, Haensch R, Beerhues L (2015). Biphenyl 4-Hydroxylases Involved in Aucuparin Biosynthesis in Rowan and Apple are CYP736A Proteins. Plant Physiol. 168: 428-442 (Impact factor 7.8).

11. Gaid MM#, Sircar D#, Müller A, Beuerle T, Liu B, Ernst L, Hänsch R, Beerhues L (2012): Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures. Plant Physiol 160: 1267-80; (IF =7.8);[# - joint first author].

10. Moon UR, Sircar D, Barthwal R, Sen SK, Beuerle T, Beerhues L, Mitra A (2015). Shoot cultures of Hoppea fastigiata (Griseb.) C.B. Clarke as potential source of neuroprotective xanthones. J Nat Med DOI 10.1007/s11418-015-0904-x (Impact factor 1.5).

09.Santos-Mecedo E#, Sircar D#, Cardoso H, Peixe A, Arnholdt-Schmitt B (2012): Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism. Plant Cell Rep. 31: 1581-90; (IF = 2.87) [[# - joint first author].

08. Sircar D, Cardoso HG, Mukherjee C, Mitra A, Arnholdt-Schmidt B (2012): Alternative oxidase (AOX) and phenolic metabolism in methyl-jasmonate-treated hairy root cultures of Daucus carota L. J. Plant Physiol 169: 657-663. (IF = 2.79) r:blue'># - joint first author].

07Sircar, D., Mukherjee, C., Beuerle, T., Beerhues, L. and Mitra A. (2011): Characterization of p-hydroxybenzaldehyde dehydrogenase - the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota. Acta Physiol Plantarum 33:2019-2024. (IF = 1.23).

06. Sircar, D. and Mitra A. (2009): Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carota 2.Confirming biosynthetic steps through feeding of inhibitors and precursors. J. Plant Physiol. 166, 1370-1380. (IF = 2.79).

05. Gaid, MM.,# Sircar, D.,# Beuerle, T., Mitra, A. and Beerhues, L. (2009): Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures. J. Plant Physiol. 166, 1343-1349. (IF = 2.79) [# - joint first author].

04. Chakraborty, D.,# Sircar, D.# and Mitra A. (2008): Phenylalanine ammonia-lyase-mediated biosynthesis of 2-hydroxy-4-methoxybenzaldehyde in roots of Hemidesmus indicus. J. Plant Physiol.  165, 1033-1040. (IF = 2.79) [#- joint first author].

03Sircar, D. and Mitra, A. (2008): Evidence for p-hydroxybenzoate formation involving enzymatic phenylpropanoid side-chain cleavage in hairy roots of Daucus carota. J. Plant Physiol. 165, 407-414. (IF = 2.79).

02Sircar, D., Dey, G. and Mitra A. (2007): A validated HPLC method for simultaneous determination of 2-hydroxy-4-methoxybenzaldehyde and 2-hydroxy-4-methoxybenzoic acid in root organs of Hemidesmus indicus. Chromatographia. 65, 349-353. (IF= 1.09).

01Sircar, D., Roychowdhury, A. and Mitra, A. (2007): Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carota. J. Plant Physiol.  164, 1358-1366.  (IF = 2.79).

 

 

 

Miscellaneous
  • Courses currently teaching: B.Tech: Plant Biotechnology (BTN 303) Cell Biology (BTN 104) M. Sc: Cell and Tissue Culture Technology (BTN 621) Plant System Physiology (BTN 642) M.Sc Project (BTN 620) M.Tech: Recombinant DNA Technology (BTN 657) New Course Developed: Plant System Physiology (BTN 642), Interactive teaching with audio-video quizes