Debabrata Sircar - Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee
Debabrata Sircar
Debabrata Sircar Associate professor debabrata.sircar@bt.iitr.ac.in
Areas of Interest
  • Non-invasive Sensors for plant quality monitoring, Prediction of fruit quality using non-invasive metabolite-based electronic sensors , Non-invasive sensors for plant disease detection, Detection of rice blast and apple disease using SMART sensor, Non-invasie mobile phone based sensor, Portable sensor for quantification of natural products from medicinal plants, ROS-omics: Comprehensive omics of apples and pear, Use of genomics & metabolomics resources for the improvement of fruit quality of Rosaceous plant, Plant metabolomics, Mass spectrometry based plant metabolomics: medicinal and fruit plants, Metabolic Pathway Engineering using SMART Plant cells, Engineering of secondary metabolic pathways in selected cis genically modified medicinal plants
Professional Background
FromPeriodPositionOrganisation
2018-01-01OngoingAssociate ProfessorIIT Roorkee
2013-01-015 years Assistant ProfessorIIT Roorkee
2011-01-012 years Post-doctoral FellowIPB, TU-Braunschweig, GERMANY
2010-01-011 year Post-doctoral FellowUniversity of Evora, PORTUGAL
Multiple Posts
FromPeriodPositionOrganisation
2015-01-01OngoingMember Secretary IBSCInstitutional Biosafety Committee
2015-01-01OngoingOfficer In-Charge AdministrationBiotechnology Department IIT Roorkee
2014-01-01OngoingOfficer In-Charge Store & PurchaseBiotechnology Department IIT Roorkee
2014-01-01OngoingMember DRC (Departmental Research Committee)Biotechnology Department IIT Roorkee
Honors and Awards
AwardInstituteYear
Institute Research Fellow (IRF) for performing outstanding research at IIT RorokeeIndian Institute of Technology Roorkee2019
DAAD Research Stays for University Academics and Scientists IIT Roorkee2015
SERB Young ScientistSERB India2013
Post-doctoral fellowshipInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2020
FCT Post-doctoral felloship (with evaluation score 5/5))Laboratory of molecular biology, University of Evora; PORTUGAL2010
DAAD FellowshipInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2008
University First, gold medal in M.Sc BotanyThe University of Burdwan, West Bengal2004
University first in B.Sc BotanyVinoba Bhave University2001
Educational Details
DegreeSubjectUniversityYear
M.ScPlant ScienceUniversity of Burdwan, West Bengal2004
Ph.D.Plant BiologyIndian Institute of Technology Kharagpur2009
FCT, Post-doctoral fellowMolecular Plant Breeding researchUniversity of Evora, Portugal2011
Post-doctoral fellowFunctional Genomics and Metabolomics researchInstitute for Pharmaceutical Biology, TU-Braunschweig, GERMANY2013
Sponsored Research Projects
TopicFunding AgencyStart DatePeriod
Functional Genomics of Phytoalexin Biosynthesis in AppleSERB Fast Track2014-016 years 6 months
Development of sensors for blast and blight diseases and stomata activity measurement in rice (O. SaICAR2019-01Ongoing
Generation of BMPR-1B gene edited goats using CRISPR/Cas technology to explore the functional role oICAR2019-01Ongoing
Assessment of the mechanism of actions of some selected Homeopathic preparations for their role in tAYUSH2018-01Ongoing
Development of an Electronic Nose For the Optimum Harvesting time and Fruit Quality in AppleICAR NASF2017-01Ongoing
Detection of Spoiled Coconut at Pre–Processing Stage by Portable Wireless Electronic Sensor.Coconut Development Board2017-01Ongoing
Assessment of the role of pterostilbene and its derivatives for the prevention of bone loss in breasDBT2016-014 years 6 months
Screening iron, zinc and carotenoid bioavailability from biofortified staple crops using coupled in DBT2015-015 years 6 months
Development of a rapid and robust high throughput reporter cell based bioassay for detection of xenoICAR NASF (as Co-PI)1918-01Ongoing
Participation in seminars
NamePlaceSponsored ByDate
Invited Talk: SEMINAR ON Emerging Trends in Bioprospecting of Phytoresources by Chemistry & BioprospForest Research Institute, DehradunFRI, DEHRADUN2019-01
Invited Talk: International conference on Conservation and sustainable use of high altitude medicinaFRI, DEHRADUNNational Medicinal Plants Board, Ministry of AYUSH2019-01
Invited Talk: 23rd National convention of society of pharmacognosy and international conferences on CSIR-National Botanical Research Institute, Lucknow (India)NBRI2020-07
Invited Talk: 6th Biennial International Conference on New Developments in Drug Discovery from NaturNational Institute of Pharmaceutical Education and Research, Punjab (India)NIPER, Mohali2020-07
Recent Advances in Ayurvedic herbal medicine- from source to manufacturing (Invited Talk)Uttarakhand Ayurved University, Dehradun, Uttarakhand (India)IIT Roorkee2020-07
XV Annual convention of ISVPT (Invited Talk)NDRI, Karnal, Haryana (India) SERB Young Scientist Grant2020-07
4th International Conf. and Exhibition on Pharmacognosy, Phytochemistry and Nat Prod (Invited Talk) Sao Paulo, BrazilIIT Roorkee2020-07
International Conference on Molecular Signaling- Recent Trends in Biosciences (Invited Talk)North Eastern Hill University, Shilong (India)IIT Roorkee2020-07
Teaching Engagements
TitleCourse CodeClass NameSemester
Cell BiologyBTN 104B.TechSpring
Plant System PhysiologyBTN 642M.ScSpring
Plant BiotechnologyBTN 303B.TechAutumn
Cell and Tissue Cult TechnologyBTN 621M.ScAutumn
Recombinant DNA TechnologyBTN 657M.TechAutumn
Projects and Thesis Supervised
Title of ProjectNames of Students
PURIFICATION, STRUCTURE ELUCIDATION AND ASSESSMENT OF ANTI-PROLIFERATIVE PROPERTY OF A NOVEL BIPHENYMr. Aman Kr. Chaudhary (M.Sc)
DEVELOPING ALTERNATIVE OXIDASE (AOX) AS A FUNCTIONAL MARKER FOR ADAPTIVE CELL-REPROGRAMMING IN APPLEMs. Kriti Juneja
EVALUATION OF THE TOTAL PHENOLIC AND FLAVONOID CONTENT AS WELL AS THE ANTIOXIDANT PROPERTY OF CALLUSMs. Pallavi Roy Chowdhury
METABOLIC STUDIES OF INDIAN MEDICINAL PLANT RUTA GRAVEOLENS FOR RAPID ISOLATION OF COMMERCIALLY IMPOMr. Sagar Tyagi
PHYTOALEXINS BIOSYNTHESIS & BIOCHEMICAL CHARACTERIZATION OF BENZALDEHYDE DEHYDROGENASE IN PyruMs Annu Verma
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Date
BIOSYNTHESIS OF BENZOATE-DERIVED BIPHENYL PHYTOALEXINS IN CELL CULTURES OF Pyrus pyrifoliaDr. Shashank Sagar SainiO2013-01
BIOCHEMICAL AND MOLECULAR ANALYSES OF PHYTOALEXIN BIOSYNTHESIS IN APPLE CELL CULTURESDr. Sarkate Amol VitthalraoO2013-01
MOLECULAR ANALYSES OF CINNAMTE CoA-LIGASE FROM PHYTOALEXIN PRODUCING APPLE CELL CULTURESDr. Deepa TeotiaO2013-01
ISOLATION AND CHARACTERIZATION OF BIOACTIVE METABOLITES FROM SELECTED MEDICINAL PLANTSMs. Kriti JunejaO2015-01
METABOLOMICS-BASED APPROACHED TO UNRAVEL HEALTH PROTECTIVE PROPERTIES OF COCONUT PRODUCTSMr. Mukund KumarO2016-01
METABOLOMICS-BASED BIOACTIVITY ASSESSMENT OF PLUMBAGO ZEYLANICAMr. Ashwani KumarO2016-01
METABOLOMICS OF ASWAGANDHA FOR CHARACTERIZATION OF VALUE ADDED NATURAL PRODUCTS.Ms. VarshaO2016-01
VOLATILE METABOLOMICS OF RIPENING ASSOCIATED METABOLITES IN APPLE.Ms KomolO2017-01
VOLATOMICS OF PAPAYA TO DETERMINE ACCURATE HARVESTING TIMEMr. Waghmode BO2017-01
FUNCTIONAL GENOMICS AND METABOLOMICS OF HEAT STRESS IN BARLEYMs. ReekuO2014-01
DEVELOPEMNT OF ELECTRONIC NOSE SENSOR FOR PRE-PROCESSING DETECTION OF SPOILED COCONUTMr. Arun KumarO2017-01
Invitations
TopicOrganistaionDate
Expert member for SEMINAR on Emerging Trends in Bioprospecting of Phytoresources. FRI, Dehradun2019-01
Linking R & D with stakeholders & EntrepreneursFRI, Dehradun2018-01
Precision Agriculture in India- Way ForwardICAR2018-01
Research Stays for University Academics and Scientists 2015TU-Braunschweig, GERMANY2015-01
Courses or Conferences Organised
Conference NameSponsored ByDate
International Conference on ‘Molecular Signaling: Recent Trends in Biomedical and Translational ResDBT, SERB, DRDO, INSA, NDRC2020-07
Indo-US conference and workshop on “Recent Advances on Structural Biology and Drug DiscoveryDBT, SERB, DRDO, INSA, NDRC and Indo-US2020-07
National International Collaboration
TopicOrganisation
Metabolomics and Functional Genomics of Rosaceous Plants.TU-BS-Germany
Metabolomics and Fluxomics analyses of plantsIIT Mandi
Metabolomics based sensor for rice disease detectionShivnadar University and BHU
Volatomics based sensorOUAT, Bhubaneswar
SMART Cell for medicinal plantsJNU
SMART sensor for quality assurance of medicinal plantNBRI LUcknow
Sub-cellular Localization of phytolaexin biosynthesis proteins in RosaceaeInstitute of Plant Biology, TU-BS, Germany
Alternative oxidase gene as functional marker candidate metabolic re-programming.University of Evora, PORTUGAL
Health protective Phytomedicine from selected Indian medicinal plantsIIT Kharagpur
Molecular breeding in apple and pearICAR-CITH, Srinagar
Metabolic flux analyses in natural product BiosynthesisIIT Mandi
Particulars of course - Courses currently teaching: B.Tech: Plant Biotechnology (BTN 303) Cell Biology (BTN 104) M. Sc: Cell and Tissue Culture Technology (BTN 621) Plant System Physiology (BTN 642) M.Sc Project (BTN 620) M.Tech: Recombinant DNA Technology (BTN 657) New Course Developed: Plant System Physiology (BTN 642)
Innovation in teaching - Interactive teaching with audio-video quizes
Books Authored
  1. Tomar V, Saini SS, Juneja K, Agrawal PK, Sircar D* (2019) Transgenic Technologies and Their Potential Applications in Horticultural Crop Improvement. Advances in Plant Transgenics: Methods and Applications (R. Sathishkumar et al. (eds.), Springer Nature Singapore Pte Ltd. https://doi.org/10.1007/978-981-13-9624-3_9
  2. Juneja K, Saini SS, Gaid M, Beerhues L, Sircar D* (2017) Production of life-saving drugs from Himalayan herbs. Sustainable Utilization of Natural Resources (Mondal, P., Dalai, A.K., eds.), CRC Press, Boca Raton, USA Chapter 11:333-352.
  3. Mitra A, Mukherjee C, Sircar D (2017) Metabolic Phytochemistry-Based Approaches for Studying Secondary Metabolism Using Transformed Root Culture Systems. In: Jha S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. Chapter 20: 513-37
  4. Nogales A, Noceda C, Ragonezi C, Cardoso H G, Campos M D, Frederico A M, Sircar D, Kumar S R,  Polidoros A, Peixe A  and Arnholdt-Schmitt B  (2015) Functional marker development from  AOX  genes requires deep phenotyping and individualized diagnosis, in Alternative respiratory pathways in higher plants (eds K. J. Gupta, L. A.J. Mur and B. Neelwarne), John Wiley & Sons, Ltd, Chichester, UK. Chapter 13.1: 273-2080.
Refereed Journal Papers

31. Juneja K, Mishra R,  Chauhan S, Gupta S, Roy P, Sircar D* (2020) Metabolite profiling and wound-healing activity of Boerhaviadiffusa leaf extracts using in vitro and in vivo models, Journal of Traditional and Complementary Medicine. (10): 52-5930.

30. Teotia D, Gaid M, Saini SS, Verma A, Yennamalli RM, Khare SP, Ambatipudi K, Mir JI,  Beuerle T, Hänsch R, Roy P, Agrawal PK, Beerhues L, Sircar D* (2019) Cinnamate: CoA‐ligase is involved in benzoate‐derived biphenyl phytoalexin biosynthesis in Malus× domestica ‘Golden Delicious' Plant Journal.  100: 1176-1192

29. Saini SS, Teotia D, Gaid MM, Sircar D* (2019) A New Enzymatic Activity from Elicitor treated Pear Cell Cultures Converting trans‐Cinnamic acid to Benzaldehyde. Physiologia Plantarum. 167: 64-74

28. Saini SS, Gaid M, Sircar D* (2019) Benzoate-CoA ligase contributes to the biosynthesis of biphenyl phytoalexins in elicitor-treated pear cell cultures. Plant cell reports. 1-9. DOI: https://doi.org/10.1007/s00299-019-02484-0

27. Tomar V, Beeurle T, Sircar D* (2019) A validated HPTLC method for the simultaneous quantifications of three phenolic acids and three withanolides from Withania somnifera plants and its herbal products. J Chromatogr B. 1124:154-60

26. Chaudhary R, Baranwal VK , Kumar R, Sircar  D,  Chauhan H (2019) Genome-wide identification and expression analysis of Hsp70, Hsp90, and Hsp100 heat shock protein genes in barley under stress conditions and reproductive development. Functional & Integrative Genomics. 19: 1007-1022

25. Varshney R, Mishra R, Das N, Sircar D, Roy P (2019) A comparative analysis of various flavonoids in the regulation of obesity and diabetes: An in vitro and in vivo study. Journal of Functional Foods. 59:194–205

24. Mishra R, Varshney R, Das N, Sircar D, Roy P (2019) Synthesis and characterization of gelatin-PVP polymer composite scaffold for potential application in bone tissue engineering. European Polymer Journal. 119:155-168

23. Varshney AL, Pathak NP, Sircar D* (2019) Design of graphene-based THz antennas. Computing, Communication and Signal Processing, 29-35

22. Liu B, Gaid M, Chizzali C, Khalil MNA, Sircar D, Reckwell, D, Beerhues  L (2019) Biphenyls and dibenzofurans are the phytoalexins of apple. Acta horticulturae. DOI: 10.17660/ActaHortic.2019.1261.37

21. Varshney AL, Pathak NP, Sircar D* (2018) Beam Steering THz Antenna Array using Graphene-based Phase Shifter 2018 IEEE Indian Conference on Antennas and Propagation (InCAP), 1-4

20. Sarkate A, Saini SS, Gaid M, Teotia D, Mir JI, Agrawal PK, Beerhues L, Sircar D*(2018) Comparative metabolomics of scab-resistant and susceptible apple cell cultures in response to scab fungus elicitor treatment. Scientific Reports. 8(1):17844

19. Sarkate A, Saini SS, Gaid M, Teotia D, Mir JI, Agrawal PK, Beerhues L, Sircar D.*(2018) Molecular Cloning and Functional Analysis of a Biphenyl Phytoalexin-specific O-methyltransferase from Apple Cell Suspension Cultures. Planta. 249(3):677-691

18. Varshney R, Varshney R, Mishra R, Gupta S, Sircar D, Roy P (2018) Kaempferol alleviates palmitic acid-induced lipid stores, endoplasmic reticulum stress and pancreatic β-cell dysfunction through AMPK/mTOR-mediated lipophagy. Journal of Nutritional Biochemistry. 57: 212-27

17. Sarkate A, Saini SS, Kumar P, Sharma AK, Sircar D* (2018) Salicylaldehyde synthase activity from Venturia inaequalis elicitor-treated cell culture of apple. J Plant Physiol. 221: 66-73.

16. Saini SS, Teotia D, Gaid M, Thakur A, Beerhues L, Sircar D* (2017) Benzaldehyde dehydrogenase-driven phytoalexin biosynthesis in elicitor treated Pyrus pyrifolia cell cultures. J Plant Physiol. 215: 154-62.

15. Sarkate A, Banerjee S, Mir JI, Roy P, Sircar D* (2017) Antioxidant and cytotoxic activity of bioactive phenolic metabolites isolated from the yeast-extract treated cell culture of apple. Plant Cell Tissue Organ Culture. 130:641-49.

14. Teotoa D, Saini SS, Gaid M, Beuerle T, Beerhues L, Sircar D * (2016) Development and Validation of a New HPLC Method for the Determination of Biphenyl and Dibenzofuran Phytoalexins in Rosaceae. J Chromatogr Sci. (2016) doi: 10.1093/chromsci /bmw019.

13. Mukherjee C, Sircar D, Chatterjee M, Das S, Mitra A (2014) Combating photooxidative stress in green hairy roots of Daucus carota cultivated under light irradiation. J Plant Physiol. 171: 179-87.

12. Sircar D, Gaid M, Chizzali C, Reckwell D, Kaufholdt D, Beuerle T, Broggini GA, Flachowsky H, Liu B, Haensch R, Beerhues L (2015). Biphenyl 4-Hydroxylases Involved in Aucuparin Biosynthesis in Rowan and Apple are CYP736A Proteins. Plant Physiology. 168: 428-442

11. Gaid MM#Sircar D#, Müller A, Beuerle T, Liu B, Ernst L, Hänsch R, Beerhues L (2012): Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures. Plant Physiology 160: 1267-80 [# - joint first author].

10.Moon UR, Sircar D, Barthwal R, Sen SK, Beuerle T, Beerhues L, Mitra A (2015). Shoot cultures of Hoppea fastigiata (Griseb.) C.B. Clarke as potential source of neuroprotective xanthones. J Nat Med DOI 10.1007/s11418-015-0904-x

9. Santos-Mecedo E#Sircar D#, Cardoso H, Peixe A, Arnholdt-Schmitt B (2012): Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism. Plant Cell Rep. 31: 1581-90; [# - joint first author].

8. Sircar D*, Cardoso HG, Mukherjee C, Mitra A, Arnholdt-Schmidt B (2012): Alternative oxidase (AOX) and phenolic metabolism in methyl-jasmonate-treated hairy root cultures of Daucus carota L. J. Plant Physiol 169: 657-663.

7. Sircar, D., Mukherjee, C., Beuerle, T., Beerhues, L. and Mitra A. (2011): Characterization of p-hydroxybenzaldehyde dehydrogenase - the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carotaActa Physiol Plantarum 33:2019-2024.

6. Sircar, D. and Mitra A. (2009): Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carota 2.Confirming biosynthetic steps through feeding of inhibitors and precursors. J. Plant Physiol. 166, 1370-1380.

5. Gaid, MM.,# Sircar, D.,# Beuerle, T., Mitra, A. and Beerhues, L. (2009): Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures. J. Plant Physiol. 166, 1343-1349. [# - joint first author].

4. Chakraborty, D.,# Sircar, D.and Mitra A. (2008): Phenylalanine ammonia-lyase-mediated biosynthesis of 2-hydroxy-4-methoxybenzaldehyde in roots of Hemidesmus indicus. J. Plant Physiol.  165, 1033-1040. [#- joint first author].

3. Sircar, D  and Mitra, A. (2008): Evidence for p-hydroxybenzoate formation involving enzymatic phenylpropanoid side-chain cleavage in hairy roots of Daucus carota. J. Plant Physiol165, 407-414.

 2. Sircar, D., Dey, G. and Mitra A. (2007): A validated HPLC method for simultaneous determination of 2-hydroxy-4-methoxybenzaldehyde and 2-hydroxy-4-methoxybenzoic acid in root organs of Hemidesmus indicusChromatographia. 65, 349-353. (IF= 1.09).

1. Sircar, D., Roychowdhury, A. and Mitra, A. (2007): Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carotaJ. Plant Physiol.  164, 1358-1366. 

 

# # # # # | # # #
Credits : Information Management Group, IIT Roorkee
Copyright © , All Rights Reserved, Institute Computer Centre, IIT Roorkee.