Prabhat Kumar Mandal - Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee
Prabhat Kumar Mandal
Prabhat Kumar Mandal Associate professor mandal.prabhat@gmail.com 91-1332-284780
Areas of Interest
  • Cancer Biology, L1 retrotransposon activity in oral cancer, Human Genetics, Jumping genes in health and disease, Diagnosis, Generation of inhouse polyclonal and monoclonal antibody, cancer diagnosis kit
Multiple Posts
FromPeriodPositionOrganisation
2021-02-09OngoingDepartment O.C ExaminationIIT Roorkee
2014-01-013 years OC Time Table IIT Roorkee
2017-01-01OngoingOC Dept. Animal Facility IIT Roorkee
2017-01-01OngoingOC Dept. Central Lab 112 IIT Roorkee
2017-01-01OngoingMember, Dept. Wast Management IIT Roorkee
2018-01-01OngoingMember, DAPC IIT Roorkee
2017-01-01OngoingOC Dept. Radioactive Facility IIT Roorkee
Educational Details
DegreeSubjectUniversityYear
B.ScPhysiologyCalcutta University, West Bengal, India1997
M.Sc.Biophysics and Molecular BiologyCalcutta University, West Bengal, India1999
Ph.D.Molecular ParasitologyJawaharlal Nehru University, New Delhi, India2006
Postdoctoral FellowHuman Genetics Dept. of Genetics,University of Pennsylvania (USA) 2010
Postdoctoral FellowHuman GeneticsJohns Hopkins University (USA)2013
Sponsored Research Projects
TopicFunding AgencyStart DatePeriod
Identification of cellular factors with a role in human LINE1 retrotranspositionDST SERB2015-112 years 11 months
Biology of Human LINE1 retrotransposonIIT Roorkee2014-033 years
Teaching Engagements
TitleCourse CodeClass NameSemester
Introduction to BiotechnologyBTN-101B. Tech. BiotechnologyAutumn
GeneticsBTN-505M.Sc BiotechnologyAutumn
Genetic EngineeringBTN-202B. Tech. BiotechnologySpring
Biotechnology Lab 1BTN-208B. Tech. BiotechnologySpring
Projects and Thesis Supervised
Title of ProjectNames of Students
A proteomics study to identify factors for re-occurrence and poor prognosis in oral cancerDr. Shahab Ali Usmani
PHDs Supervised
TopicScholar NameStatus of PHDRegistration Date
Investigating L-1 retrotransposon encoded ORF1 protein expression as a potential biomarker in OSCCDr. ABHIJEET SINGH (MS, DNB)A2020-08
Investigating Human L1 Retrotransposon Activity in Oral Squamous Cell Carcinoma PatientsSavita BudaniaA2013-01
Studies on Human LINE-1 Retrotransposon Encoded ORF2 ProteinSofia PilliA2014-01
Investigating Human Long Interspersed Element 1 (LINE 1) Activity in Health and DiseasesDebpali SurA2015-01
Analysis of Tissue specific L 1 retrotransposon expression and L1 RNP characterizationKoel MukherjeeO2016-01
Municipal wastewater treatment plants as a reservoir of antibiotic resistanceJahnavi KO2015-01
Biology of Human LINE1 retrotransposonsMr. Sujoy KunduO2020-08
Special Lectures Delivered
TitlePlaceDate
Jumping genes become active in critical brain areas as we age AIIMS, New Delhi2018-01
Role of mobile genetic elements in human health and diseaseDept of Biophysics,Molecular Biology and Bioinformatics, Calcutta University, WB, India2020-01
L1 retrotrasposon activity in normal human brain and in oral cancerNational Institute of Biomedical genomics (NIBMG), WB, India2020-01
Extensive LINE-1 promoter hypomethylation and ORF1p expression in oral squamous cell carcinomaAIIMS, Rishikesh2018-01
The influence of age on jumping gene activity in human brain.JNU, New Delhi2017-01
L1 retrotransposon activity in cancer.JNU, New Delhi2016-01
National International Collaboration
TopicOrganisation
Detection of Biomarkers and actionable target in Oral Squamous Cell Carcinoma (OSCC) Prof. S P Agarwal, HOD, Dept. of Surgical Oncology, AIIMS, Rishikesh, India
Investigating L1 retrotransposon activity in the Human brainProf. Anita Mahadevan, Dept. of Neuropathology, NIMHANS, Bangalore, India
Biology of Human L1 retrotransposonProf. Haig Kazazian, Johns Hopkins University, USA
Investigation of L1 retrotransposon activity in Entamoeba histolyticaProf. Sudha Bhattacharya, SES. Jawaharlal Nehru University New Delhi, India
Referred Journal papers

2021

19. Mukherjee KSur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. BMC cancer (Nature Springer) 2021, accepted. (Impact Factor 3.2). 

18. Mukherjee KSur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. biorxiv.org doi:https://doi.org/10.1101/2020.11.24.395285. 

17. Kaur D, Agrahari M, Singh S, Mandal PK, Bhattacharya A, Bhattacharya S*. Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain. Plasmid (Elsevier),2021,114,102560. doi: 10.1016/j.plasmid.2021. (Impact Factor 2.85). 

2020

16.  Budania S, Sur D, Nangal J, Pilli S, Mukherjee K, Biswas M, Prasad R, Saxena S, and Mandal PK*. LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma. Cancer Genetics (Elsevier), 2020, 244: 21-29. (*Corresponding author). (Impact Factor-3.1). 

2018

15. Kurasam J, Sihag P, Mandal PK, Sarkar S.  Presence of Fluoroquinolone Resistance with Persistent Occurrence of GyrA Gene Mutations in a Municipal Wastewater Treatment Plant in India. Chemosphere. 2018, 211: 817-825. DOI: 10.1016/j..2018.08.011 (Impact Factor-4.42). 

2017

14. Singh B, Verma A, Pooja, Mandal PK, Datta S. A biotechnological approach for degradation of inhibitory compounds present in lignocellulosic biomass hydrolysate liquor using Bordetella sp. BTIITR. Chemical Engineering Journal, 2017, 328, 519–526. DOI: 10.1016/j.cej.2017.07.059 (Impact Factor-10.6). 

13. Kumara R, Anjlika, Ratnam A, Kumar S, Bala M, Sur D, Narang S, Singh UP, Mandal PK*, Ghosh S*. Organometallic ruthenium nitrosyl obtained by C-H bond activation: Photo-induced delivery of nitric oxide (NO) and NO mediated anti-proliferation activity studies.  European Journal of Inorganic Chemistry. 2017, 5334-5343.DOI:10.1002/ejic.201700839 (Impact Factor-2.5). 

12. Sur D, Kustwar RK, Budania S, Mahadevan S, Hancks DC, Yadav V, Shankar SK and Mandal PK*. Detection of the LINE-1 retrotransposon RNA-binding protein ORF1p in different anatomical regions of the human brain. BMC Mobile DNA (Nature Springer). 2017; 8:17. doi: 10.1186/s13100-017-0101-4  (Impact Factor-5.1). 

2016

11. Mandal PK and Kazazian HH. Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells. Methods in Molecular Biology (Springer) on "Retrotransposons and Transposons, 2016.1400, 299-310. 

Post Doc Publications: 

10. Mandal PK, Ewing AE, Hancks DC, Kazazian HH Jr.  Enrichment of processed pseudogene transcripts in L1- ribonucleoprotein particles. Human Molecular Genetics. 2013. 22. 3730-3748. 

9. Hancks DC, Mandal PK, Cheung LE, Kazazian HH Jr. The minimal active human SVA retrotransposon requires Only the 5'-hexamer and Alu-like domains. Molecular and Cellular Biology. 2012.32:4718-4726. 

8. Hancks DC, Goodier JL*, Mandal PK*, Cheung LE, Kazazian HH Jr. Retrotransposition of marked SVA elements by human L1s in cultured cells. Human Molecular Genetics. 2011.20:3386-3400. (*Equal Contribution). 

7. Goodier JL, Mandal PK, Zhang L, Kazazian HH Jr. Discrete subcellular partitioning of human retrotransposon RNAs despite a common mechanism of genome insertion. Human Molecular Genetics. 2010.19: 1712-1725. 

6. Mandal PK, Kazazian HH Jr. Snapshot: Vertebrate Transposons. Cell. 2008.135: 192.  

5. Babushok DV, Ohshima K, Ostertag EM, Chen X, Wang Y, Mandal PK, Okada N, Abrams CS,  Kazazian HH Jr, A novel testis ubiquitin binding protein, S5a-like, arose by exon-shuffling in hominoids. Genome Research. 2007.17: 1129-1138. 

Ph.D Publications: 

4. Yadav VP, Mandal PK, Bhattacharya A, Bhattacharya S. SINE chimeras are formed at high frequency during induced retrotransposition in vivo. Nature communications. 2012 DOI:10.1038. 

3. Yadav VP, Mandal PK, Rao DN, Bhattacharya S. Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal repeat retrotransposon EhLINE1. FEBS J. 2009, 276:7070-7082. 

2. Mandal PK, Rawal K, Ramaswamy R, Bhattacharya A, Bhattacharya S. Identification of insertion hot spots for non-LTR retrotransposons: computational and biochemical application to Entamoeba histolytica. Nucleic Acids Res. 2006, 34(20): 5752-5763. 

1. Mandal PK, Bagchi A, Bhattacharya A, Bhattacharya S. An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease. Eukaryotic Cell. 2004, 3(1): 170-179.  

Books Authored

1. H.H. Kazazian, Jr., M.d.c. Seleme, D.V.Babushok, D.M. Ostertag, M.R. Vetter, P.K. Mandal. Human diversity and L1 Retrotransposons Biology: Creation of New Genes and Individual Variation in Retrotransposition Potential. Retrotransposition, Diversity and the Brain. Book Chapter, Yera 2008,Pages 43-52.

 

Patents

Polyclonal human LINE-1 ORF2p antibody and method thereof(Indian Patent Application No. 201911028257 dated July 15, 2019). Prabhat K Mandal, Koel Mukherjee, Debpali Sur.

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